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pCRISPR2_IRdist_scram

Plasmid

#162319

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

The distal Upstream Motif site is scrambled

Promoter

pBAD

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pPV-C1-crRNA-cloning-EF1a-BA

Plasmid

#204623

Purpose

piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2-del344-380_T

Plasmid

#147623

Purpose

Mammalian Expression of DmNot2-del344-380

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del344-380 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pVAX1-VSVG-INDIANA2

Plasmid

#196623

Purpose

Express VSV-G protein in mammalian cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

VSV-G

Expression

Mammalian

Mutation

WT

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ8-CadR-Pcadh-LacZ

Plasmid

#191623

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadR

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/MACROD1

Plasmid

#172578

Purpose

For generation of FlpIn lines with untagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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KHBD00523

Plasmid

#39623

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11966 (CG11966 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S70D

Plasmid

#13623

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Ser 70 to Asp

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
Td-CBE-NW

Plasmid

#240623

Purpose

Cytidine base editor with narrowed editing window

Depositor

Tingting Jiang

Article

Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

Insert

TadA-8e (E27R, N46L, N108Q, M151C, Q154Y)-linker-nCas9-P2A-UGI

Use

CRISPR

Expression

Mammalian

Mutation

TadA-8e (E27R, N46L, N108Q, M151C, Q154Y), Cas9-P…

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ phiC31 integrase

Plasmid

#216237

Purpose

For the overexpression of phiC31 integrase

Depositor

Hyunju Ro

Article

Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

Insert

integrase from phage φC31

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV IE94

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDL026_HA-rb_IgG_LC_15F11

Plasmid

#193623

Purpose

HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-HA_15F11 rabbit light chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pX330-EN479

Plasmid

#86234

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse rosa26 locus

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-15

Plasmid

#228971

Purpose

For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-15

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV4a-SIRT2-Flag

Plasmid

#102623

Purpose

Expresses full length SIRT2 isoform 1 in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

Sirtuin 2 (SIRT2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-5

Plasmid

#228965

Purpose

For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-14

Plasmid

#228994

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-14

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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myc-AP4 Epsilon

Plasmid

#127623

Purpose

N-terminal myc-tagged AP4 epsilon subunit

Depositor

Josef Kittler

Article

Ivankovic et al Autophagy. 2019 May 29:1-17. doi: 10.108

Insert

AP-4 complex subunit epsilon-1 (AP4E1 Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pQCascade-array1

Plasmid

#140623

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCD510B attp BSD

Plasmid

#216236

Purpose

Lentiviral vector, For genome integration of an attP PhiC13 recognition site

Depositor

Hyunju Ro

Article

Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

Insert

blasticidin S deaminase

Use

Lentiviral

Expression

Mammalian

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBX_RPL7a_smRuby2_HA

Plasmid

#236087

Purpose

Constitutively encodes RPL7a-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting the 60S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPL7a-smRuby2-HA (RPL7A Synthetic, Human)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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