Addgene

We narrowed to 721 results for: RPL

Showing: 501 - 520 of 721 results

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pvLEA22mer_mutant_3_mCh-2xFKBP (pBS1123)

Plasmid

#185318

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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PvLEA4_repeats_k5_1_mCh-2xFKBP (pBS1122)

Plasmid

#185317

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k5_1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pvLEA22mer_mutant_10_mCh-2xFKBP (pBS1121)

Plasmid

#185316

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PvLEA4_repeats_k3_26_mCh-2xFKBP (pBS1118)

Plasmid

#185313

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_26

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRLCon1288-1666

Plasmid

#31448

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRLCon183-221_850-1207

Plasmid

#31449

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragments (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRLCon183-221mut2_850-1207

Plasmid

#31450

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragments (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

mut2 in balancer

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRLCon1-449_850-1207

Plasmid

#31451

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragments (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-shC

Plasmid

#21963

Depositor

Lynda Chin

Article

Ji et al Nature. 2007 Aug 16. 448(7155):807-10.

Insert

si NEDD9 (NEDD9 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
IF-GFP-ATRX

Plasmid

#45444

Purpose

ATRX expression vector

Depositor

Michael Dyer

Article

Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

Insert

ATRX (ATRX Human, Mouse)

Use

Tags

GFP and HA

Expression

Bacterial and Mammalian

Mutation

isoform 2, missing codon E124

Promoter

PCMV

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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pALS2-sfGFP WT

Plasmid

#197575

Purpose

Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
M. alvus Pyl-tRNA (6)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

araC and lpp

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBK- Ma PylRS WT

Plasmid

#197573

Purpose

Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Insert

M. alvus PylRS WT

Use

Tags

Expression

Bacterial

Mutation

Promoter

GlnS (constitutive)

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Rab35 S22N inactive

Plasmid

#47426

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 S22N (RAB35 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

S22N inactive

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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GFP_Rab35 Q67L active

Plasmid

#47425

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 Q67L (RAB35 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Q67L active

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-Rab35 Q67L active

Plasmid

#47428

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 Q67L (RAB35 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Q67L active

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pAcBac1-Ma-sfGFP WT

Plasmid

#197567

Purpose

Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
M. alvus Pyl-tRNA (6) (4x copies)

Use

Tags

V5-His6

Expression

Mammalian

Mutation

Promoter

CMV and U6/H1

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-Rab35 Q67L

Plasmid

#47434

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 Q67L (RAB35 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Q67L active

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-Rab35 S22N inactive

Plasmid

#47429

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 S22N (RAB35 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S22N inactive

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
Myc-Rab35 S22N

Plasmid

#47435

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 S22N (RAB35 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

S22N inactive

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-shD

Plasmid

#21964

Depositor

Lynda Chin

Article

Ji et al Nature. 2007 Aug 16. 448(7155):807-10.

Insert

si NEDD9 (NEDD9 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only