We narrowed to 2,803 results for: cdna expression plasmid

1. pCDNA3.0_mitoNanobody IRES EGFP

   Plasmid

   #136621

   Purpose

   Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   NTOM20-SNAP-Tag-EnhancerNb (IRES EGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.0_LynNanobody IRES EGFP

   Plasmid

   #136624

   Purpose

   Plasmid encoding outer inner membrane localization of the Enhancer nanobody GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   Lyn11-SNAP-Tag-EnhancerNb (IRES EGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

   Plasmid

   #189562

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   contains aa 1-213

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

   Plasmid

   #110376

   Purpose

   Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Tags

   FLAG and tGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_27F3_hFc

   Plasmid

   #199619

   Purpose

   Encodes DARPin-hFc 27F3 for mammalian expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-hFc 27F3
   

   Use

   Tags

   hFc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1_27B3_hFc

   Plasmid

   #199618

   Purpose

   Encodes DARPin-hFc 27B3 for mammalian expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-hFc 27B3
   

   Use

   Tags

   hFc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-MS2-HA-HshnRNPM-del243-615_Z

   Plasmid

   #148128

   Purpose

   Mammalian Expression of HshnRNPM-del243-615

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPM-del243-615 (HNRNPM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutations compared to the sequence of …

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-LYRa11-RBD-8h

   Plasmid

   #161822

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T324-D522)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-RsSHC014-RBD-8h

   Plasmid

   #161826

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Rs7327-RBD-8h

    Plasmid

    #161823

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-Rs4084-RBD-8h

    Plasmid

    #161825

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4084

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-Rs4231-RBD-8h

    Plasmid

    #161824

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4231

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T320-D518)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA 3.1 BS (-) N protein

    Plasmid

    #188448

    Purpose

    SARS CoV-2 N protein expression

    Depositor

    Ajit Chande

    Article

    Mishra et al Life Sci Alliance. 2022 Mar 16;5(7). pii

    Insert

    SARS-CoV-2 N protein
    

    Use

    Tags

    Strep-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.3_CoV2_501V2

    Plasmid

    #170449

    Purpose

    Express SARS-CoV-2 501V2 (South Africa strain / beta strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 501V2 pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Yuan et al Science. 2021 Aug 13;373(6556):818-823.

    Insert

    SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, 501V2 (B.1.351))
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D6…

    Promoter

    CMV

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.3_CoV2_P1

    Plasmid

    #170450

    Purpose

    Express SARS-CoV-2 P.1 (Brazil / gamma) strain spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 P.1 pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Yuan et al Science. 2021 Aug 13;373(6556):818-823.

    Insert

    SARS-CoV-2 P.1 spike D18 (S SARS-CoV-2, P.1 strain)
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

    Promoter

    CMV

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid

    #145145

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    

    Use

    Tags

    8his tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3_QLuc

    Plasmid

    #183260

    Purpose

    Express QLuc in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

    Insert

    QLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-sACE2(WT)-8his

    Plasmid

    #149268

    Purpose

    Mammalian expression plasmid for his-tagged sACE2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-SARS-1-RBD-8h

    Plasmid

    #161821

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T320-D518)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-sACE2-WT(732)

    Plasmid

    #154098

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 (untagged)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge