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Plasmid#183997PurposeOptically controlled eDrTrkB expression in mammalian cellsDepositorInserteDrTrkB (NTRK2 Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ-441
Plasmid#195465PurposeLentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: HP1a-ZF3DepositorInsertpSFFV - HP1a - ZF3 - ERT2 - SMASh
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pGAP T-
Plasmid#161503PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Non targeting control NTC)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM0652
Plasmid#129013PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJBL7346
Plasmid#217370PurposesfGFP reporter for synthetic transcriptional variant of Pca rhtB ZTP riboswitchDepositorInsertPca rhtB synthetic transcriptional riboswitch sfGFP reporter
UseTagsExpressionMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE-miRFP703_PGK-Puro
Plasmid#216667PurposeExpresses tTa-inducible miRFP703, with puromycin resistanceDepositorInsertsmiRFP703
PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PHGDH-int2
Plasmid#188682Purposecontrol sgRNADepositorInsertsgPHGDH intron control (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PHGDH-int1
Plasmid#188681Purposecontrol sgRNADepositorInsertsgPHGDH intron control (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PHGDH-int3
Plasmid#188683Purposecontrol sgRNADepositorInsertsgPHGDH intron control (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenLox_U6 p53
Plasmid#59360PurposeLentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.DepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterCAG and mouse U6Available sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-miniXon_Luciferase
Plasmid#174660PurposePlasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.DepositorInsertminiXon cassette
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-Xon_Luciferase
Plasmid#174659PurposePlasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.DepositorInsertXon cassette
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-idCas9-vpr
Plasmid#89985Purposeinducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locusDepositorInsertdCas9-VPR
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry::LANS4
Plasmid#60785PurposeControl of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear TranslocationDepositorInsertLANS4
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHW533 (Peft-3::cGAL-N::let-858 3'UTR)
Plasmid#107132PurposecGAL-N driver under the control of C. elegans eft-3 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans eft-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW531 (Peft-3::cGAL-C::let-858 3'UTR)
Plasmid#107136PurposecGAL-C driver under the control of C. elegans eft-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
UseTagsExpressionWormMutationPromoterC. elegans eft-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW530 (Prab-3::cGAL-N::let-858 3'UTR)
Plasmid#107131PurposecGAL-N driver under the control of C. elegans rab-3 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans rab-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW522 (Prab-3::cGAL-C::let-858 3'UTR)
Plasmid#107130PurposecGAL-C driver under the control of C. elegans rab-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
UseTagsExpressionWormMutationPromoterC. elegans rab-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)
Plasmid#107133PurposecGAL-N driver under the control of C. elegans hsp-16.41 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans hsp-16.41 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV mTurquoise2-shadowG tandem
Plasmid#157774PurposeProduces a fusion between mTurquoise2 and shadowG. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneUseTagsmTurquoise2 and shadowGExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only