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pUBC(k)-AT1G73430-mKOk

Plasmid

#224835

Purpose

Plasmid for expression of AT1G73430.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT1G73430.1 (AT1G73430 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC(k)-AT1G77730-mKOk

Plasmid

#224836

Purpose

Plasmid for expression of AT1G77730.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT1G77730.1 (AT1G77730 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC(b)-AT1G22850-mKOk

Plasmid

#224831

Purpose

Plasmid for expression of AT1G22850.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT1G22850.1 (AT1G22850 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pFPO-His-mTurquoise2_T203Y

Plasmid

#191456

Purpose

Bacterial isolation of mTurquoise2 with a T203Y mutation

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

mTurquoise2_T203Y

Tags

His

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Lck-mScarlet-I

Plasmid

#98821

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lck

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Lck-mTurquoise2

Plasmid

#98822

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lck

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-tdT-P2A-Mxra8

Plasmid

#242477

Purpose

AAV vector expressing Mxra8 and tdTomato

Depositor

Justus Kebschull

Article

Kim et al bioRxiv 2025.06.25.661405; doi: https://

Inserts

Mxra8 (Mxra8 Mouse)
tdTomato

Use

AAV

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_sfGFP-TurboID

Plasmid

#240226

Purpose

Gateway entry clone with TurboID tagged with superfolder GFP (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

sfGFP-TurboID

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

Plasmid

#191457

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-jRCaMP1b

Plasmid

#191468

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

jRCaMP1b

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a>WAChR_1m-G-Soma-E-HA-P2A-mScarlet; WPRE3-BGH polyA; KanR

Plasmid

#247728

Purpose

Constitutive bicistronic expression via P2A cleavage: {WAChR-1m}-{Kir2.1 Golgi tag}-{Kv2.1 soma tag}-{Kir2.1 ER tag}-{HA tag} + {mScarlet}

Depositor

Alexander Naka

Article

Tose et al bioRxiv 2025.09.12.675947; doi: https://

Insert

WAChR_1m-G-Soma-E-HA-P2A-mScarlet

Use

AAV

Tags

mScarlet

Expression

Mammalian

Mutation

S122L/F240A

Promoter

EF1a

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSBbi-MTS-FAP-GFP

Plasmid

#242990

Purpose

Expresses MTS-FAP-GFP in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

MTS-dH6.2-GFP

Use

Sleeping beauty

Tags

GFP

Expression

Mammalian

Promoter

EF-1⍺

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBbi-FAP-GFP-KDEL

Plasmid

#242991

Purpose

Expresses FAP-GFP-KDEL in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

dH6.2-GFP-KDEL

Use

Sleeping beauty

Tags

GFP

Expression

Mammalian

Promoter

EF-1⍺

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-MS2-RecT-dCas9-BSD

Plasmid

#226108

Purpose

Using ef-1a promoter, expresses dCas9 and RecT protein that intended to be tethered via MS2 gRNA hairpin to dCas9, and Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol 8 (2025). https://doi.org/10

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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M6667

Plasmid

#219772

Purpose

MoClo-compatible Level 0 vector carrying terminator ORCA3

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

Terminator ORCA3

Use

Synthetic Biology

Expression

Plant

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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M6570

Plasmid

#219773

Purpose

MoClo-compatible Level 0 vector carrying promoter pORCA3

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

Promoter ORCA3

Use

Synthetic Biology

Expression

Plant

Promoter

pORCA3

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pNK3625

Plasmid

#219770

Purpose

MoClo-compatible Level 0 vector carrying promoter pWRKY70

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

Promoter WRKY70

Use

Synthetic Biology

Expression

Plant

Promoter

pWRKY70

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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M6597

Plasmid

#219771

Purpose

MoClo-compatible Level 0 vector carrying terminator WRKY70

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

Terminator WRKY70

Use

Synthetic Biology

Expression

Plant

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-V5

Plasmid

#240240

Purpose

Expression of EGFP-V5 under control of copper-inducible promoter.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

EGFP-V5

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_HA-spGFP11

Plasmid

#240224

Purpose

Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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