We narrowed to 4,691 results for: pcr

1. pCRII-Topo Tmtc4

   Plasmid

   #45869

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   TMTC4 in situ probe (Tmtc4 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   nt 1220-1798 on BC031368

   Promoter

   Available since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pCR8-DJ1-E64D

   Plasmid

   #29356

   Depositor

   Mark Cookson

   Article

   Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

   Insert

   DJ1 (PARK7 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Glu64Asp

   Promoter

   Available since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

3. 27 pCR plcg 1 CDS

   Plasmid

   #13058

   Depositor

   Nathan Lawson

   Article

   Lawson et al Genes Dev. 2003 Jun 1. 17(11):1346-51.

   Insert

   plcg1 (plcg1 Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 18, 2010

   Availability

   Academic Institutions and Nonprofits only

4. PCR4-Shh::lacZ-H11

   Plasmid

   #139098

   Purpose

   enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

   Depositor

   Len Pennacchio

   Article

   Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Shh

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPaint-mScarlet-PuroR [M1G]

   Plasmid

   #171809

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR-U6-gRNA-TRE3G-TdTomato

   Plasmid

   #192658

   Purpose

   A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   TdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, TRE3G

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR2.1 Clover-LMNA Donor

   Plasmid

   #122508

   Purpose

   Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

   Depositor

   Graham Dellaire

   Article

   Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

   Insert

   Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
   

   Use

   Homology repair template plasmid (donor plasmid)

   Tags

   Clover

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCROPseq-VEX-optimized-scaffold

   Plasmid

   #203321

   Purpose

   Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR-U6-gRNA-miniCMV-TdTomato

   Plasmid

   #192656

   Purpose

   A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   TdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, miniCMV

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR-HOT-Clover-BlastR

    Plasmid

    #138569

    Purpose

    Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    Clover-BlastR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR-blunt-TOPO-FNDC5

    Plasmid

    #35970

    Depositor

    Bruce Spiegelman

    Article

    Bostrom et al Nature. 2012 Jan 11;481(7382):463-8. doi

    Insert

    fibronectin type III domain containing 5 (Fndc5 Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2012

    Availability

    Academic Institutions and Nonprofits only

12. PCR4-Hsp68::lacZ-H11

    Plasmid

    #139099

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Hsp68

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPaint-mScarlet-BlastR [M1G]

    Plasmid

    #171814

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL3617 (pCRISPR(BbsI)_Pse)

    Plasmid

    #200902

    Purpose

    Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCRISPR (entry)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-tadDE-PcrA M6-UGI

    Plasmid

    #221236

    Purpose

    Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    tadDE-PcrA M6-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR8-attL3-pegRNA-attL2

    Plasmid

    #213052

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    35S-CmYCLV-AtU6

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR8-attL1-pegRNA-attR3

    Plasmid

    #213051

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    35S-CmYCLV-AtU6

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCR4-Hsp68::eGFP-H11

    Plasmid

    #211941

    Purpose

    dual-enSERT-1 eGFP vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter)

    Depositor

    Evgeny Kvon

    Article

    Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSP68

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-AID-PcrA-UGI

    Plasmid

    #221220

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPEY-GAL-Nat-ADE2

    Plasmid

    #232103

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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