166,012 results

1. pET3a-hUBA1

   Plasmid

   #63571

   Purpose

   Expression of human UBA1 tagged with His

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBA1 (UBA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Isoform 2 (1018 amino acids)

   Promoter

   T7

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. JD150

   Plasmid

   #229485

   Purpose

   Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   E2A, VA RNA I, E4orf6, L4-22k/33k
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTTH+H1.4

   Plasmid

   #157796

   Purpose

   Expression of human linker histone H1.4

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H1.4 (H1-4 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiGuide Puro-P2A-EGFP

   Plasmid

   #137729

   Purpose

   For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (AAV1)

   Viral Prep

   #100834-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (#100834). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 plasmid DNA. CamKII-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CaMKII

   Tags

   None

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

7. SPLICS Mt-ER Long P2A

   Plasmid

   #164107

   Purpose

   Detect the long Mitochondria-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Mt-ER Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCMV_Bless

   Plasmid

   #207255

   Purpose

   Mammalian expression of Baboon Endogenous Retrovirus env protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   BaboonEndogenousRetrovirus_Rless_env
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcdna3.1 hciap1

   Plasmid

   #8311

   Depositor

   Jon Ashwell

   Article

   Li et al Nature 2002 Mar 21;416(6878):345-7.

   Insert

   cIAP1 (BIRC2 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-Ubiquitin

    Plasmid

    #18712

    Purpose

    Mammalian expression of human ubiquitin with HA tag

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

    Insert

    ubiquitin (UBB Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The HA-Ubiquitin insert is from the UBB gene. The…

    Promoter

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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