We narrowed to 22,823 results for: Sis

1. pICH86966::AtU6p::sgRNA:NbVPE1a/b

   Plasmid

   #223218

   Purpose

   Expresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoter

   Depositor

   Tsepo Tsekoa

   Article

   Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

   Insert

   NbVPE1a/b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6 promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IP-muGFP-hATG2A mLIR

   Plasmid

   #215506

   Purpose

   Expresses GFP tagged human ATG2A with the mutation in LC3 interacting region.

   Depositor

   Noboru Mizushima

   Article

   Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

   Insert

   Autophagy related 2A (ATG2A Human)
   

   Use

   Retroviral

   Tags

   muGFP

   Expression

   Mammalian

   Mutation

   P656R (natural variant with no functional relevan…

   Promoter

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE FL NAP1 STOP C' EGFP IRES Blast

   Plasmid

   #179102

   Purpose

   Expresses untagged full length NAP1

   Depositor

   Alicia Pickrell

   Article

   Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

   Insert

   Nak associated protein 1 (AZI2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-N'-FLAG-HA-NAP1S318A

   Plasmid

   #195410

   Purpose

   Expresses FLAG-HA tagged human NAP1 with S318A point mutation

   Depositor

   Alicia Pickrell

   Article

   Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

   Insert

   Nak associated protein 1 (AZI2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Serine 318 turned to Alanine

   Promoter

   Available Since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJ205

   Plasmid

   #162676

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C224A, C529A; codon optimized for expression in E…

   Promoter

   T7/lac

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCJ198

   Plasmid

   #162671

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   F495I; codon optimized for expression in E. coli …

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCJ196

   Plasmid

   #162669

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   S225A; codon optimized for expression in E. coli …

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCJ204

   Plasmid

   #162677

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C224H, C529F; codon optimized for expression in E…

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCJ201

   Plasmid

   #162674

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C224W, C529SS; codon optimized for expression in …

   Promoter

   T7/lac

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. DOT1L-CC3

    Plasmid

    #111754

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #3 (residues 613-661)

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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