We narrowed to 13,844 results for: cas9 genes

1. LC3B sgRNA

   Plasmid

   #207556

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the LC3B locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CACTGAATACCAGCGCCTAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MDC1 KO sgRNA

   Plasmid

   #207103

   Purpose

   pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GGTGTAACGTGGAGCCAGTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DNA-PKcs N-terminal sgRNA

   Plasmid

   #207093

   Purpose

   pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   AGCGGGACTCGGCGGCATGG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ULK1 sgRNA

   Plasmid

   #207559

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CCAGCCAGGCCAGAAAGGTC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ATG9A sgRNA

   Plasmid

   #207557

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CACTGAATACCAGCGCCTAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p458 VQR

   Plasmid

   #101727

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   VQR

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. px458_Conc2

   Plasmid

   #134449

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. WIPI2 sgRNA

   Plasmid

   #207554

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the WIPI2 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CGCGCGCCCAGCCATGAACC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMB1610_pRR-Puro

   Plasmid

   #65853

   Purpose

   Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

   Depositor

   Marc Bühler

   Article

   Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

   Insert

   split puromycin N-acetyltransferase coding sequence
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. px458_Conc3

    Plasmid

    #134450

    Purpose

    Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ATG16L1 sgRNA

    Plasmid

    #207563

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    GCAGCAAGTGACATGTCGTC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. px459V2.0_Conc3

    Plasmid

    #134458

    Purpose

    Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 53BP1 N-terminal sgRNA

    Plasmid

    #207091

    Purpose

    pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GGGGAGCAGATGGACCCTAC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ATG5 sgRNA

    Plasmid

    #207555

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG5 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    AACTTGTTTCACGCTATATC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ULK1 KO sgRNA

    Plasmid

    #207561

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CCCGCCTGCGCCATGGAGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ATM N-terminal sgRNA

    Plasmid

    #207089

    Purpose

    pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ATCATTAAGTACTAGACTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pECNUS_MP01

    Plasmid

    #194350

    Purpose

    CRISPR-Cas9 multiplexing acceptor clone with BpiI cutter

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    pcoCas9, FAST-Red
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTJV1Sc2-tetA

    Plasmid

    #166982

    Purpose

    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    CspRecT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pVanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm

    Plasmid

    #159636

    Purpose

    Donor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    GCaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lenticrispr-wt-ltr-puro

    Plasmid

    #173428

    Purpose

    Expresses Cas9 and guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. px459 EQR

    Plasmid

    #101732

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VQR

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pICSL1P3_AtU626Ter26

    Plasmid

    #191771

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pICSL11197

    Plasmid

    #191784

    Purpose

    SpCas9 expression in plants

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    AtUbi10::Cas9 +13 introns-T-E9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLentiCRISPRv1.linker_KO

    Plasmid

    #164688

    Purpose

    For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    miR-144~451 linker CRISPR KO gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCUX1.1.0-gDNA

    Plasmid

    #112434

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1

    Depositor

    Kevin White

    Insert

    CUX1 (CUX1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RNF168 C-terminal sgRNA

    Plasmid

    #207100

    Purpose

    pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GAGATGCACAAAGTAAGGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. RIF C-terminal sgRNA

    Plasmid

    #207096

    Purpose

    pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AATACTAAATAGAATTTTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. REV7 C-terminal sgRNA

    Plasmid

    #207095

    Purpose

    pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GCTCATAAAGGCAGCTGAGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKDC.109

    Plasmid

    #227189

    Purpose

    T7 IPTG-driven Cas9

    Depositor

    Seth Shipman

    Article

    Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

    Insert

    SpyCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSCL391

    Plasmid

    #219501

    Purpose

    Express Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequence

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Eco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SHLD3 N-terminal sgRNA

    Plasmid

    #207094

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTACTGCAGAATGACTACAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. NBS1 C-terminal sgRNA

    Plasmid

    #207092

    Purpose

    pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TAAAAAGGAGAAGATAACTG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. RNF169 C-terminal sgRNA

    Plasmid

    #207099

    Purpose

    pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ACACTTCATTAGGTGCTACT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SHLD1 N-terminal sgRNA

    Plasmid

    #207101

    Purpose

    pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ATGGCAGGACTATGGCAGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ATM C-terminal sgRNA

    Plasmid

    #207097

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTTCTAAAGGCTGAATGAAA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SHLD3 KO sgRNA #1

    Plasmid

    #207105

    Purpose

    pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    CGCTATCAAGATTTATACCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. ATG13 sgRNA

    Plasmid

    #207558

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG13 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    ggaaactgatctcaattccc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SHLD2 N-terminal sgRNA

    Plasmid

    #207098

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTTTTATCAGAAATCATGAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HaloTag KO sgRNA

    Plasmid

    #207102

    Purpose

    pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GTCGATGTTGGTCCGCGCGA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MDC1 N-terminal sgRNA

    Plasmid

    #207090

    Purpose

    pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GTATCCTTCCCAGATCATGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge