We narrowed to 63,016 results for: NAM

1. pRS315-48nt-telo-I-SceI

   Plasmid

   #67623

   Purpose

   telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

   Depositor

   Kurt Runge

   Article

   Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

   Inserts

   3' gal1 unique sequence
   ura4 (ura4 Fission Yeast)
   48 bp telomere repeats
   hph
   

   Use

   Yeast cen/ars plasmid

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ02B2Gm_AE

   Plasmid

   #66063

   Purpose

   MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   FACS Controls
   

   Use

   Synthetic Biology

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPTXcGMPRELUC

   Plasmid

   #68503

   Purpose

   cyclic nucleotide reporter for bacteria or plant cells

   Depositor

   Helen Irving

   Article

   Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

   Insert

   OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
   

   Tags

   luciferase

   Expression

   Bacterial and Plant

   Mutation

   3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

   Promoter

   OPTX promoter with cGMPRE

   Available Since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-ABKAR-Kras

   Plasmid

   #84637

   Purpose

   Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
   

   Tags

   6xHis, C-terminal targeting sequence from Kras, C…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCarCBAE

   Plasmid

   #117755

   Purpose

   Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

   Depositor

   Greg Bokinsky

   Article

   Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

   Insert

   Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
   

   Expression

   Bacterial

   Promoter

   PLac(UV5)

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNIA-CEN-Venus-FLAG-LINXa4-Bre1

   Plasmid

   #73618

   Purpose

   Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   Venus
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A

   Promoter

   ADH1

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS315-0nt-telo-I-SceI

   Plasmid

   #67624

   Purpose

   telomere cassette for telomere formation system containing 0 bp of S. pombe telomere repeats

   Depositor

   Kurt Runge

   Article

   Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

   Inserts

   3' gal1 unique sequence
   ura4 (ura4 Fission Yeast)
   telomere cassette and I-SceI cut site
   hph
   

   Use

   Yeast cen/ars plasmid

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ERKsub(TA)DD-Venus-NES

   Plasmid

   #84631

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   ERKsub(TA)DD-Venus-NES
   

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Mutated target Thr residue to Ala.

   Promoter

   CMV

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ02B2Gm_EF

   Plasmid

   #66064

   Purpose

   MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   FACS Controls
   

   Use

   Synthetic Biology

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc-HT-Dmp53KRKR

    Plasmid

    #85699

    Purpose

    expression of Drosophila p53 KRKR mutant in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    Dmp53 (p53 Fly)
    

    Tags

    His

    Expression

    Insect

    Mutation

    K26R K302R

    Promoter

    Ac5

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-AMPKsub-YPet-Kras

    Plasmid

    #84638

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-Kras
    

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST53-Arl13b20

    Plasmid

    #40876

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted Arl N-terminal domain; added stop codon

    Promoter

    CMV

    Available Since

    Nov. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pTXB1-TP1

    Plasmid

    #160084

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein, no linker

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTXB1-TP2

    Plasmid

    #160085

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. ROZA-XL YF

    Plasmid

    #64195

    Purpose

    Fluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F mutated control sequence

    Depositor

    Annemarie Lellouch

    Article

    Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.

    Insert

    ROZA-XL YF
    

    Expression

    Mammalian

    Mutation

    ROZA-XL tyrosine phosphorylation site mutated to …

    Promoter

    CMV

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pOPTXGARE

    Plasmid

    #68542

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with GARE (AT1G33440 Mustard Weed)
    

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    5x GARE inserted 190 bp 5' pf ATG (GARE = ta…

    Promoter

    OPTX promoter with GARE

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNB785

    Plasmid

    #60164

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-mCherry-LKB1(S431A)

    Plasmid

    #84641

    Purpose

    mCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation site

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(S431A) (STK11 Synthetic, Human)
    

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    PKA phosphorylation site Serine 428 mutated to Al…

    Promoter

    CMV

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNB787

    Plasmid

    #60152

    Purpose

    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNB789

    Plasmid

    #60166

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    RNR1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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