We narrowed to 21,041 results for: Vars

1. pcDNA3.1-GFP-Flag-UTR

   Plasmid

   #183235

   Purpose

   To express FLAG tagged eGFP

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   EGFP
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Thy1 promoter construct

   Plasmid

   #20736

   Depositor

   Joshua Sanes

   Article

   Feng et al Neuron. 2000 Oct . 28(1):41-51.

   Insert

   Thy1 promoter (Thy1 Mouse)
   

   Expression

   Mammalian

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3xFLAG-MKL1

   Plasmid

   #11978

   Purpose

   For mammalian expression of human MKL1 with a 3x FLAG tag

   Depositor

   Ron Prywes

   Article

   Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

   Insert

   MKL1 (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N579S T716A

   Promoter

   CMV

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJE53

   Plasmid

   #194153

   Purpose

   sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   sgRNA with mrfp control guide sequence
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. gfp-Arkadia/RNF111

   Plasmid

   #112228

   Purpose

   Expresses GFP-tagged Arkadia in mammalian cells

   Depositor

   Vasso Episkopou

   Article

   Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

   Insert

   (Arkadia)Rnf111 (Rnf111 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. MC177VSG221RNAi

   Plasmid

   #59730

   Purpose

   For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

   Depositor

   Gloria Rudenko

   Article

   Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

   Insert

   VSG221 RNAi fragment
   

   Use

   RNAi

   Promoter

   opposing T7 promoters

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. BPK1520_puroR

   Plasmid

   #173901

   Purpose

   SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

   Depositor

   Ronald Cohn

   Article

   Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV/U6

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNW-sfGFP(SPS6)

   Plasmid

   #133814

   Purpose

   Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(SPS6)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Expression

   Bacterial

   Promoter

   Ppta

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-noPS

   Plasmid

   #177943

   Purpose

   Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   eGFP
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEY14

    Plasmid

    #191059

    Purpose

    acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-5(orf) (acr-5 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS_VP30_MARV

    Plasmid

    #135472

    Purpose

    Mammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    VP30
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    AG

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDV24_AAV2_DART-VADAR_iRFP720-sensor

    Plasmid

    #200411

    Purpose

    AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    DART VADAR sensor
    

    Use

    AAV and Synthetic Biology

    Tags

    MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

    Plasmid

    #159808

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-GFAP104-melanopsin-mCherry

    Plasmid

    #122630

    Purpose

    AAV-mediated expression of melanopsin-mCherry under the GFAP promoter

    Depositor

    Edward Boyden

    Article

    Mederos et al Glia. 2019 May;67(5):915-934. doi: 10.10

    Insert

    melanopsin-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    GFAP104

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-UbC-FKRP-EF1a-BSD

    Plasmid

    #205150

    Purpose

    Express UbC-driven FKRP and EF-1a driven BSD

    Depositor

    Monkol Lek

    Insert

    Fukutin-related protein (FKRP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PRS_8-jRGECO1a

    Plasmid

    #210399

    Purpose

    AAV-vector for fluorescent calcium measurements in the red spectrum from noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    jRGeco1a
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    PRSx8

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-HLA-A2_GFP

    Plasmid

    #85161

    Purpose

    HLA.A2_GFP fusion with 2 glycines in between

    Depositor

    Tone Fredsvik Gregers, Sébastien Walchli

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Gateway entry vector

    Tags

    EGFP

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ypet-PBD

    Plasmid

    #105290

    Purpose

    To measure Rac or Cdc42 activity by binding and FRET analysis

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    aa encoding 65-150

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-SlugABE-NNG-P2A-puro

    Plasmid

    #214354

    Purpose

    Expresses SlugABE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG and puromycin resistance genes
    

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Nanog-2A-mCherry

    Plasmid

    #59995

    Purpose

    Inserts mCherry immediately downstream of the Nanog coding region

    Depositor

    Rudolf Jaenisch

    Article

    Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2A-mCherry

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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