We narrowed to 11,578 results for: VARS

1. Talin1(ΔR1-10)-mCherry

   Plasmid

   #212007

   Purpose

   Expression of mouse talin1 (1-490 + 1974-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

   Insert

   Talin1(ΔR1-10)-mCherry (Tln1 Mouse)
   

   Use

   Tags

   mCherry fluorescent protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLINE1mORF1-ΔORF2-scFvFc

   Plasmid

   #206023

   Purpose

   The base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR

   Plasmid

   #200639

   Purpose

   Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUBQ10:LYK5_At-3xHA

   Plasmid

   #202192

   Purpose

   Express Arabidopsis thaliana LYK5 gene under UBQ10 promoter

   Depositor

   Gitta Coaker

   Article

   Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

   Insert

   LYSM-CONTAINING RECEPTOR-LIKE KINASE 5 (LYK5 Mustard Weed)
   

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNMSB96

   Plasmid

   #199316

   Purpose

   MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

   Depositor

   Michael Krieg

   Article

   Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

   Insert

   flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mTagBFP2 from pJJR81, C. elegans codon optimized …

   Promoter

   flp-18

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1

   Plasmid

   #197421

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2

   Plasmid

   #197422

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

   Plasmid

   #197423

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

   Plasmid

   #197424

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

    Plasmid

    #197425

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2/shCol1a1/Scarlet_Seq1.2

    Plasmid

    #201404

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT2/shCol1a1/Scarlet-Seq1.1

    Plasmid

    #201401

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2/Col1a1/GFP4_Seq 1.1

    Plasmid

    #201400

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-In18runon-PTPN22

    Plasmid

    #195377

    Purpose

    A human PTPN22 mutant (intron 18-runon) in Gateway pDONR221

    Depositor

    Patrick Concannon

    Article

    Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

    Insert

    PTPN22 (PTPN22 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Intron 18-runon PTPN22

    Promoter

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-Myc-PSK2 (K57A)

    Plasmid

    #197118

    Purpose

    Expression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

    Insert

    TAOK1 (K57A) (TAOK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Changed K 57 to A

    Promoter

    SV40

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-In18runon-PTPN22

    Plasmid

    #195379

    Purpose

    Mammalian expression of a human PTPN22 mutant (intron 18-runon) with FLAG tag

    Depositor

    Patrick Concannon

    Article

    Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

    Insert

    PTPN22 (PTPN22 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Intron 18-runon PTPN22

    Promoter

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-Ex1-17-PTPN22

    Plasmid

    #195375

    Purpose

    Mammalian expression of a human PTPN22 mutant (exon 1-17) with FLAG tag

    Depositor

    Patrick Concannon

    Article

    Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

    Insert

    PTPN22 (PTPN22 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    PTPN22 mutant containing exon 1 to 17

    Promoter

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGADT7-γ1

    Plasmid

    #197078

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-1 γ1 (Ap1g1 Mouse)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. P2061

    Plasmid

    #186299

    Purpose

    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10

    Inserts

    LexA-HBD-B112
    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    2xLexop-minimalCyc1 and PACT1

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-U6-DR30(sapI)-CMV-intron-hfCas13d-pA

    Plasmid

    #195865

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Yes: GTGGAATACATTACCAACGTGGTGTACGTG

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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