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FB013

Plasmid

#119710

Purpose

TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231360

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231361

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pscAAV.T7-SP6-BC(p11-14)-CAG-EGFP-SV40pA-T7

Plasmid

#231350

Purpose

Self complementary AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231351

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231354

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231355

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7

Plasmid

#231356

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CAG-EGFP-W3SL-BC(p1-10)

Plasmid

#231347

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-CAG-EGFP-W3SL-T7

Plasmid

#231348

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pscAAV.BC(p1-6)-CAG-EGFP-SV40pA-BC(p1-6)

Plasmid

#231349

Purpose

Self complementary AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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Nuclear Pro-Code Vector Kit

Plasmid Kit

#1000000197

Purpose

The Nuclear Pro-Code Vector Kit is a set of 75 lentiviral vector plasmids, each with a unique barcode (Pro-Code) and gRNA cloning site.

Depositor

Brian Brown

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERP2-RUNX2

Plasmid

#192926

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

RUNX2 (RUNX2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NR5A1

Plasmid

#192915

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of NR5A1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

NR5A1 (NR5A1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FOXL2

Plasmid

#192914

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2 (FOXL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Mkk7-JNK2 fusion-pcw107

Plasmid

#64618

Purpose

Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Map2k7 linker MAPK9

Use

Lentiviral

Tags

FLAG

Expression

Mutation

mouse-human fusion

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
CROP-seq-puro-v2-no-scaffold

Plasmid

#228521

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Inserts

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-mTurquoise2-v2-no-scaffold

Plasmid

#228522

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Insert

mTurquoise2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MAFF

Plasmid

#192917

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of MAFF

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

MAFF (MAFF Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-YBX1

Plasmid

#192904

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of YBX1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

YBX1 (YBX1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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