We narrowed to 41,163 results for: gats

1. p3E-SGTAP no-pA

   Plasmid

   #82599

   Purpose

   3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   SBP-TEV-2x protein G
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-R4R3-3xmRuby-N

   Plasmid

   #113754

   Purpose

   Gateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mRuby2-mRuby2-mRuby2
   

   Use

   CRISPR; Gateway entry vector

   Tags

   3xmRuby2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-CMV-Flag-BRSK2_IDG-K

   Plasmid

   #135279

   Purpose

   Gateway destination clone of BRSK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

   Depositor

   Ben Major

   Insert

   Flag-BRSK2 (BRSK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUSE-B_P2rP4

   Plasmid

   #97185

   Purpose

   For fusion gene cloning. Recombine Gene B into this Gateway Entry Vector

   Depositor

   Kenneth Scott

   Article

   Lu et al Cancer Res. 2017 May 16. pii: canres.274

   Type

   Empty backbone

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. BII-C3HTK

   Plasmid

   #133388

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3E-FRB(PLF)-HA no-pA

   Plasmid

   #82597

   Purpose

   3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FRB-HA (no pA)
   

   Use

   Tags

   Expression

   Mutation

   K2095P, T2098L, W2101F

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ908

   Plasmid

   #44474

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, PuroR, [4-3] Gateway
   

   Use

   Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-DEST-R1R2-pNeo

   Plasmid

   #141057

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Neomycin resistance gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCVL SFFV Sbf-Sal linker

   Plasmid

   #50434

   Purpose

   Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLY134

    Plasmid

    #130962

    Purpose

    Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK TT 3xFLAG LacZ neo

    Plasmid

    #83105

    Purpose

    Lentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

12. p3E-Dam-myc no-pA

    Plasmid

    #82600

    Purpose

    3' entry vector containing myc epitope tag fused to C-terminus of E. coli DNA adenine methyltransferase without pA for DamID

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    Dam-Myc (no pA)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1

    Plasmid

    #141025

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-L1-PGK-Neo-L2

    Plasmid

    #141007

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Neomycin resistance gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. KJ101: pMVP (R4-R3) osTir1

    Plasmid

    #121742

    Purpose

    pMVP R4-R3 entry plasmid, contains osTir1 for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    osTir1
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    9x myc epitope tag

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. IG603: pMVP (L1-L4) TRE promoter

    Plasmid

    #121691

    Purpose

    pMVP L1-L4 entry plasmid, contains TRE promoter for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. IG401: pMVP (L1-R5) RIP

    Plasmid

    #121694

    Purpose

    pMVP L1-R5 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    RIP
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAM5187

    Plasmid

    #120086

    Purpose

    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid.

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Type

    Empty backbone

    Use

    Shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUHD Cdk2 DN HA

    Plasmid

    #27651

    Depositor

    Greg Enders

    Article

    Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

    Insert

    Cdk2 (CDK2 Human)
    

    Use

    Tags

    HA (YDVPDYA)

    Expression

    Mammalian

    Mutation

    D146N (Dominant Negative)

    Promoter

    Available since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

20. p663-UBC-miniTurbo-V5-CAMK1D_IDG-K

    Plasmid

    #158167

    Purpose

    Gateway destination clone of CAMK1D (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CAMK1D (CAMK1D Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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