We narrowed to 8,454 results for: gnal

1. 3xNLS-NLP-cMyc-cMyc AspCas12a

   Plasmid

   #182122

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc AspCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LIC-Z-YFP

   Plasmid

   #153544

   Purpose

   perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Tags

   Venus YFP

   Expression

   Mammalian

   Mutation

   with mCherry in LIC-Z replaced by YFP

   Promoter

   CMV

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. RCAS-sgATG7

   Plasmid

   #75228

   Purpose

   An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

   Depositor

   Noor Gammoh

   Article

   Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

   Insert

   Autophagy Related 7 (Atg7 Mouse)
   

   Use

   Retroviral

   Promoter

   U6

   Available Since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+)-RIα(D/D+linker)-EGFP

   Plasmid

   #181842

   Purpose

   EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-(D/D+linker)EGFP (PRKAR1A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains only residues 12-112 of PKA RIα.

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRAF_S29A_pcDNA3.1

   Plasmid

   #86506

   Purpose

   Expresses CRAF_S29A in mammalian cells [Edit]

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   CRAF (RAF1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S29A

   Promoter

   CMV

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-FLAG-FLCN (C terminus)

   Plasmid

   #72296

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

   Insert

   FLCN C-terminus (FLCN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains only C terminus of FLCN as defined in PM…

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pPGK-T7/2-CD44v8-10 (-cyt)

   Plasmid

   #137822

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v8-10 (-cyt) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region

   Promoter

   PGK

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1-HA-MEK1 C121S

   Plasmid

   #53164

   Purpose

   Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   C121S

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-FLAG-hDIXDC1 S592A

   Plasmid

   #61223

   Purpose

   Mammalian expression vector encoding long isoform of human DIXDC1

   Depositor

   Reuben Shaw

   Article

   Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

   Insert

   human DIXDC1 (DIXDC1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed Serine 592 to Alanine

   Promoter

   CMV

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET28A-p53 (1-73) (P to A)

    Plasmid

    #62069

    Purpose

    Expression of human p53 (1-73) (all P to A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; all Prolines changed …

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pEF5/FRT/DEST-3xHA/mGli2 P5-6D

    Plasmid

    #51254

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 923,956 mutated to Asp

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pSUPER-retro-puro-tetO-Mek1-shRNA

    Plasmid

    #53179

    Purpose

    Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (Map2k1 Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

13. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAN19-3xFLAG-TIR1-NOSt

    Plasmid

    #108545

    Purpose

    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    3xFLAG

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSico shMAPKAPK3 human

    Plasmid

    #85661

    Purpose

    expression of shRNA targeting human MAPKAPK3

    Depositor

    Julien Sage

    Article

    Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

    Insert

    MAPKAPK3 (MAPKAPK3 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pECE beta1 R760A

    Plasmid

    #50511

    Depositor

    Kenneth Yamada

    Article

    Pankov et al J Biol Chem. 2003 May 16;278(20):18671-8

    Insert

    integrin beta1 (ITGB1 Human)
    

    Expression

    Mammalian

    Mutation

    R760A

    Promoter

    SV40

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. LP696

    Plasmid

    #90177

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1289-1826

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    aa residues 1289-1826

    Promoter

    CMV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. RON6-bio

    Plasmid

    #47785

    Purpose

    Expresses enzymatically monobiotinylated full-length RON6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RON6
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin replaced…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSP1-mp53AS-6xMUT

    Plasmid

    #20904

    Depositor

    Stefano Piccolo

    Article

    Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

    Insert

    p53AS (Trp53 Mouse)
    

    Expression

    Mammalian

    Mutation

    point mutation (Ser/Thr-Ala) of the 6 N-terminal …

    Available Since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

20. GFP-Drosha270-1374delNLS2

    Plasmid

    #62532

    Purpose

    N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    truncated human Drosha mutant (DROSHA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-269 and nuclear localizatio…

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only