We narrowed to 166,096 results for: addgene

1. MSCV PIG Pu.1

   Plasmid

   #66982

   Purpose

   Retroviral expression plasmid for mouse Pu.1 expression; containing bicistronic IRES-GFP

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Spi1 (Spi1 Mouse)
   

   Use

   Retroviral

   Promoter

   LTR

   Available Since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Sst22-GFP

   Plasmid

   #172312

   Purpose

   SST interneuron-restricted gene regulatory element GRE22, to drive GFP expression in SST+ interneurons

   Depositor

   Michael Greenberg

   Article

   Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

   Insert

   Sst22
   

   Use

   AAV

   Promoter

   pB-Globin

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCOM-CD63-COM

   Plasmid

   #138350

   Purpose

   Expressing COM-CD63-COM fusion protein in mammalian cells

   Depositor

   Baisong Lu

   Article

   Yao et al J Extracell Vesicles. 2021 Mar;10(5):e12

   Insert

   COM-CD63-COM fusion protein
   

   Use

   CRISPR and Lentiviral

   Tags

   COM is fused to the N and C termini of human CD63

   Expression

   Mammalian

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. VCP F12.4 gRNA

   Plasmid

   #90939

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human VCP

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   VCP (Guide Designation F12.4)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. VCP F11.4 gRNA

   Plasmid

   #90938

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human VCP

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   VCP (Guide Designation F11.4)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. XLone-BSD SPI1

   Plasmid

   #179514

   Purpose

   Tunable and temporal expression control of SPI1

   Depositor

   Xiaoping Bao

   Article

   Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3GS Promoter

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ABCG1_pCSdest

   Plasmid

   #53952

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   ABCG1 (ABCG1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pDR-GFPuniv

   Plasmid

   #46085

   Purpose

   In vivo recombination assay fluorescent reporter

   Depositor

   Raymond Monnat

   Article

   Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

   Insert

   DR-GFPuniv reporter
   

   Use

   Recombination reporter plasmid

   Expression

   Mammalian

   Mutation

   E5G and K163R mutations in EGFP relative to wild-…

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. HUNK_pCSdest

   Plasmid

   #53809

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   HUNK (HUNK Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

10. EZH2 E10.3 gRNA

    Plasmid

    #90685

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EZH2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    EZH2 (Guide Designation E10.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE-puro LAP2

    Plasmid

    #15712

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    LAP2 (CEBPB Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pRRL_U2AF1_S34Y_mCherry

    Plasmid

    #84018

    Purpose

    expression of mutant U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    S34Y

    Promoter

    PGK

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pFA6a-yomRuby3-SpHIS5

    Plasmid

    #204287

    Purpose

    For C-terminal tagging using yeast optimized mRuby3

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pFA6-yomRuby3-SpHIS5
    

    Tags

    yomRuby3

    Expression

    Yeast

    Promoter

    none

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUW-TetO-tdTomato-cNLS

    Plasmid

    #138532

    Purpose

    Lentiviral inducible expression of tdTomato with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    tdTomato
    

    Use

    Lentiviral

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CamKIIa:smFP-TVA-RGV

    Plasmid

    #202594

    Purpose

    Expresses TVA receptor, rabies oG and “spaghetti monster” fluorescent protein in mature excitatory neurons.

    Depositor

    Oleksandr Shcheglovitov

    Article

    Chiola et al Mol Psychiatry. 2021 Feb 8. pii: 10.1038

    Inserts

    smFP
    TVA
    RGV
    

    Use

    Lentiviral

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2-CAG-fGFP

    Plasmid

    #108714

    Purpose

    Transposon donor plasmid for long-term expression of membrane targetted GFP

    Depositor

    Madeline Lancaster

    Article

    Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

    Insert

    EGFP
    

    Tags

    6xHis and farnesylation signal

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Hmga2 3'UTR m7 luciferase (Luc-m7)

    Plasmid

    #14788

    Depositor

    David Bartel

    Article

    Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

    Insert

    Luc-m7 (Hmga2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    m7 mutant (see article and author's map for …

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-pMecp2-SaCas9-pU6-sgRNA

    Plasmid

    #159173

    Purpose

    Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL12009

    Plasmid

    #68257

    Purpose

    Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter and 5UTR Ubiquitin (Zea mays)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMBEC8

    Plasmid

    #187599

    Purpose

    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    nCas9-BEC-UGI; cas6f, gfp
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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