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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,480 results for: nls

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Showing: 5221 - 5240 of 31480 results

  2. HcRed1-Nucleus-7

    Plasmid
    #55843
    Purpose
    Localization: Nucleus, Excitation: 588, Emission: 618
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    HcRed1-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid
    #107319
    Purpose
    Expresses SpCas9 fused to dSaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107319-plasmid-map-sequence-id-204682

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  7. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid
    #114089
    Purpose
    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized FnCas12a
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114089-plasmid-map-sequence-id-220552

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  9. pDN-D2irTNG4kwh

    Plasmid
    #44722
    Depositor
    Gabor Balazsi
    Publication
    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    pCMV-D2i promoter
    htetR::NLS::EGFP
    Use
    Synthetic Biology; Expression regulator/reporter;…
    Tags
    Rabbit β-globin intron II and WPRE
    Expression
    Mammalian
    Mutation
    Initiator motif (Inr) displaced relative to pCMV-…
    Promoter
    pCMV-D2i
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    44722-plasmid-map-sequence-id-61263

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  10. dCas9-NLS-gs10-cGR2176

    Plasmid
    #188258
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Publication
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cGR2176
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    188258-plasmid-map-sequence-id-377988

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  11. dCas9-NLS-gs10-cDHFR174

    Plasmid
    #188256
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Publication
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cDHFR174 (DHFR Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188256-plasmid-map-sequence-id-374594

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  16. pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE

    Plasmid
    #223287
    Purpose
    A complete OptoMBP cassette for dissolution of FUS-CHOP condensates.
    Depositor
    Jared Toettcher
    Publication
    Brumbaugh-Reed et al Nat Commun. 2024 Aug 7;15(1):6717. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    myc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
    Use
    Synthetic Biology; Piggybac transposase insertabl…
    Expression
    Mammalian
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    223287-plasmid-map-sequence-id-442312

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  20. pT22iuasvApoWChe(#488)

    Plasmid
    #184108
    Purpose
    cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Publication
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    4xUAS
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184108-plasmid-map-sequence-id-363970

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Showing: 5221 - 5240 of 31480 results