We narrowed to 11,563 results for: Vars;

1. VIR_sgRNA

   Plasmid

   #186661

   Purpose

   VIR sgRNA plasmid

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   VIR sgRNA Plasmid (vir Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pST_CAHS1_RAMVA_108-197_noLink (pBS0654)

   Plasmid

   #185198

   Purpose

   For the mammalian expression of the tardigrade protein CAHS1_RAMVA_108-197_noLink. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CAHS1_RAMVA_108-197_noLink
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pST_CAHS1_RAMVA_108-197 (pBS0652)

   Plasmid

   #185196

   Purpose

   For the mammalian expression of the tardigrade protein CAHS1_RAMVA_108-197. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CAHS1_RAMVA_108-197
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pST_CAHS2_RAMVA_94-170 (pBS0295)

   Plasmid

   #185161

   Purpose

   For the mammalian expression of the tardigrade protein CAHS2_RAMVA_94-170. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CAHS2_RAMVA_94-170
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pST_MAHS_RAMVA_81-236 (pBS0289)

   Plasmid

   #185159

   Purpose

   For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   MAHS_RAMVA_81-236
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAT079

   Plasmid

   #180509

   Purpose

   Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   DpbCasX
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. DSUP_RAMVA_1-208_mCh-2xFKBP (pBS1116)

   Plasmid

   #185311

   Purpose

   For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DSUP_RAMVA_1-208
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP]

   Plasmid

   #153542

   Purpose

   AAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeQ-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α1.1

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1α1.1-FLEX-rc [ChromeT-GFP]

   Plasmid

   #153541

   Purpose

   AAV-mediated expression of ChromeT-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeT-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α1.1

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Equalizer-L plasmid with onboard mCherry cassette

    Plasmid

    #169735

    Purpose

    Equalizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM10 with rat Δ192Annexin A11

    Plasmid

    #164498

    Purpose

    For bacterial expression of rat His-tagged Δ192Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat Δ192Annexin A11 (Anxa11 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N-terminal truncation

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM10 with rat Δ188Annexin A11

    Plasmid

    #164497

    Purpose

    For bacterial expression of rat His-tagged Δ188Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat Δ188Annexin A11 (Anxa11 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N-terminal truncation

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Syn-FLEX-rc [ChromeT-GFP]

    Plasmid

    #153539

    Purpose

    AAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW_0007

    Plasmid

    #102839

    Purpose

    Binary vector containing Sr22 PI573523 allele driven by Sr33 promoter, Sr33 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 PI573523 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Sr33 promoter

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBW_0002

    Plasmid

    #102834

    Purpose

    Binary vector containing Sr22 Schomburgk allele driven by Sr33 promoter, Sr33 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 Schomburgk (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Sr33 promoter

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBW_0003

    Plasmid

    #102835

    Purpose

    Binary vector containing Sr22 Schomburgk allele driven by Sr33 promoter, Sr22 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 Schomburgk (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Sr33 promoter

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBW_0004

    Plasmid

    #102836

    Purpose

    Binary vector containing Sr22 PI190945 allele driven by Sr33 promoter, Sr33 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 PI190945 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Sr33 promoter

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBW_0005

    Plasmid

    #102837

    Purpose

    Binary vector containing Sr22 PI573523 allele driven by maize ubiquitin promoter, Sr22 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 PI573523 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBW_0006

    Plasmid

    #102838

    Purpose

    Binary vector containing Sr22 Schomburgk allele driven by Sr33 promoter, Sr33 promoter (undomesticated)

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 Schomburgk (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Sr33 promoter

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUPD2 TNos:Luc:5'UTR (GB1499)

    Plasmid

    #160580

    Purpose

    Reversed sequences of the NOS terminator, Firefly Luciferase CDS and 5' UTR of 35S promoter

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    Luc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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