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pSuper.Retro.Puro-WDR5 shRNA 2

Plasmid

#59975

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Publication

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pGLB2 5'3'

Plasmid

#49346

Purpose

luciferase reporter with Bmp2 promoter (1702 bp) and Bmp2 3'UTR (2015 bp)

Depositor

Melissa Rogers

Publication

Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8

Inserts

Bmp2 distal promoter (Bmp2 Mouse)
Bmp2 3' UTR (Bmp2 Mouse)

Use

Luciferase

Tags

luciferase

Promoter

-

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
NTSP-PC1

Plasmid

#41575

Depositor

Thomas Weimbs

Publication

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

Myc/Myc/His

Expression

Mammalian

Mutation

4106-4218 of FLS PC1

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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CTS-PC1

Plasmid

#41576

Depositor

Thomas Weimbs

Publication

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7 and myc/myc/his

Expression

Mammalian

Mutation

FLS (4184-4303)

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-ACVR1B

Plasmid

#23567

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ACVR1B (ACVR1B Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-CDK10

Plasmid

#23652

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK10 (CDK10 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PCTK1

Plasmid

#23754

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PCTK1 (CDK16 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-DGUOK

Plasmid

#23864

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DGUOK (DGUOK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
MEIS1B-pSP65

Plasmid

#21019

Depositor

Corey Largman

Publication

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1B (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pFastBac K-Ring1A

Plasmid

#1994

Depositor

Robert Kingston

Publication

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Ring1A (Ring1 Mouse)

Expression

Insect

Mutation

Kozak before start codon

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pDM002-sgRNA-lenti-ZeoR

Plasmid

#200060

Purpose

Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

Depositor

Britt Glaunsinger

Publication

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

Bleomycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-KA

Plasmid

#64981

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Publication

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-FOLD6

Plasmid

#186547

Purpose

FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

FOLD6

Tags

6xHis

Expression

Bacterial

Mutation

mGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…

Promoter

araB

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmReNL

Plasmid

#182970

Purpose

Stably express PalmReNL in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Publication

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmReNL

Expression

Mammalian

Mutation

N/A

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LT2_7_OxPro

Plasmid

#177709

Purpose

Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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GPX8 rescue PLAK1

Plasmid

#161517

Purpose

Rescues the expression of GPX8 in GPX8 plenti-CRISPR KO cells

Depositor

Yoav Shaul

Publication

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

glutathione peroxidase 8 (GPX8 Human)

Use

Lentiviral

Tags

3X Flag

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNCST-AausFP2

Plasmid

#129500

Purpose

Expresses AausFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10

Plasmid

#127129

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K24R- please see depositor comments

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSNAP-FtnA

Plasmid

#98282

Purpose

mammalian expression of SNAP-FtnA

Depositor

Mike Heilemann

Publication

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FtnA

Tags

SNAPf-tag

Expression

Mammalian

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open]

Plasmid

#71809

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Publication

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]

Expression

Insect

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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