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pCDH-CRY2-mCh-superPLDx30

Plasmid

#188988

Purpose

A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)

Depositor

Jeremy Baskin

Publication

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CRY2 and mCherry

Expression

Mammalian

Mutation

K109R, P245A, G328S, G381V, G429D

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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p-mCherry2-sgMUC4

Plasmid

#198399

Purpose

Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

Depositor

Sarah McClelland

Publication

Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

Insert

MUC4 sgRNA (MUC4 Human)

Expression

Mammalian

Promoter

U6

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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SNAP-CLC-EGFP

Plasmid

#193579

Purpose

Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

SNAP-tag and mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_mApple-SEPT7 Gmut2

Plasmid

#180317

Purpose

mammalian expression of human SEPT7 Gmut2 fused to mApple

Depositor

Manos Mavrakis

Publication

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

mApple

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple

Plasmid

#180323

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple

Depositor

Manos Mavrakis

Publication

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG1_zeo

Plasmid

#184910

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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C413-E45: attB4-hr5-ie1p>-attB1r

Plasmid

#162930

Purpose

Gateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPV

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV ie1 promoter with hr5 enhancer

Use

Synthetic Biology

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pscMS2(WTx2)-EGFP

Plasmid

#140258

Purpose

MS2CP-dependent translational repression

Depositor

Hirohide Saito

Publication

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

Two MS2CP-binding sites embedded in scaffold

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R364

Plasmid

#138234

Purpose

Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)

Depositor

Baojun Wang

Publication

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NGK).Cth-Ter*.(SEL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-GGA(EAAAK)2AGG

Plasmid

#137104

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Publication

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GGA(EAAAK)2AGG

Use

Synthetic Biology

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-CD86-mApple

Plasmid

#133860

Purpose

Mammalian expression of CD86 N-terminally tagged with EGFP and C-terminally tagged with mApple

Depositor

Dave Piston

Publication

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

CD86 (CD86 Human)

Tags

EGFP and mApple

Expression

Mammalian

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPagP

Plasmid

#112125

Purpose

Bacterial expression of outer membrane protein PagP, wild type

Depositor

Karen Fleming

Publication

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

PagP (pagP E. coli)

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLSU4mg10

Plasmid

#126079

Purpose

Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_1

Plasmid

#124912

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Publication

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Arl6ip6

Plasmid

#120235

Purpose

Expresses V5-tagged Arl6ip6 in lentiviral vector

Depositor

Larry Gerace

Publication

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Arl6ip6 (Arl6ip6 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT W63A

Plasmid

#90246

Purpose

Expresses YFP-HA-DAT W63A mutant in mammalian cells

Depositor

Alexander Sorkin

Publication

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated W63 to A

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YFP-HA-DAT R60A

Plasmid

#90245

Purpose

Expresses YFP-HA-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Publication

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated R60 to A

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GST-RP2

Plasmid

#86072

Purpose

A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

RP2 (RP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPc

Plasmid

#87166

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Publication

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

YFPc

Promoter

35s

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_1

Plasmid

#68807

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Publication

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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