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Showing: 5281 - 5300 of 5415 results

  2. pBI121::chitRatioGFP:LAAQ

    Plasmid
    #240451
    Purpose
    Expression of indicator protein fusion (modified ratiometric pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. See Resource Information.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of chitinase signal, soluble modified ratiometric pHluorin and low affinity Aequorin (D119A)
    Expression
    Bacterial and Plant
    Mutation
    Ratiometric GFP (ratiometric pHluorin) (PMID 9671…
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available Since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240451-plasmid-map-sequence-id-485323

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  8. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid
    #186427
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Rb7
    eGFP
    Use
    Synthetic Biology; Binary vector for escherichia …
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186427-plasmid-map-sequence-id-369354

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  13. pSLIK-NFLAG-hEWSR1G511A-CMyc

    Plasmid
    #50734
    Purpose
    Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 G511A (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Mutation
    Changed Gly 511 to Ala
    Promoter
    TRE
    Available Since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50734-plasmid-map-sequence-id-481096

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  15. pSLIK-NFLAG-hEWSR1P552L-CMyc

    Plasmid
    #50736
    Purpose
    Produces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 P552L (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Mutation
    Changed Pro 552 to Leu
    Promoter
    TRE
    Available Since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. Endy/Shearwin pOSIP plasmid set

    Plasmid Kit
    #1000000035
    Purpose
    Cloning and assembly of DNA constructs for chromosomal integration in E. coli.
    Depositor
    Drew Endy
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Clonetegration
    Available Since
    July 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. CRISPRainbow Kit for Multiplex Labeling

    Plasmid Kit
    #1000000079
    Purpose
    Plasmids using dCas9 and gRNA scaffolds that bind fluorescent proteins to label multiple chromosomal loci.
    Depositor
    Thoru Pederson
    Application
    Genome Editing
    Vector Type
    Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Available Since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. The Expanded EasyClone-MarkerFree toolkit

    Plasmid Kit
    #1000000190
    Purpose
    Expansion of the EasyClone-MarkerFree Vector Set (#1000000098) with eight additional chromosomal loci for the stable integration of heterologous DNA into Saccharomyces cerevisiae by CRISPR/Cas9.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  19. pCut plasmid toolkit

    Plasmid Kit
    #1000000118
    Purpose
    Plasmids for integrating into well-characterized chromosomal loci to program gene expression in S. cerevisiae.
    Depositor
    Aindrila Mukhopadhyay
    Application
    Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 5281 - 5300 of 5415 results