We narrowed to 32,140 results for: Eng

1. pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

   Plasmid

   #119074

   Purpose

   Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   ubb:MCP-p65-HSF1-2a-Cerulean
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish ubiquitin promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

   Plasmid

   #119075

   Purpose

   Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   ubb:Com-KRAB-2a-Cerulean
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish ubiquitin promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVC-Ds-DrU6a:sgRNA-com-Ds

   Plasmid

   #119077

   Purpose

   To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:2xCom-sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish U6a promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVC-Ds-DrU6a:sgRNA-MS2-Ds

   Plasmid

   #119076

   Purpose

   To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:2xMS2-sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish U6a promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EE-mut SV40 luc

   Plasmid

   #8460

   Depositor

   Bob Weinberg

   Article

   Geng et al Oncogene 1996 Mar 21;12(6):1173-80.

   Insert

   human cyclin E promoter fragment, mutant (CCNE1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   E2F sites I, II, and III mutated.

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pRKB134_GI-DUX4-intron2(-)

   Plasmid

   #83968

   Purpose

   to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

   Depositor

   Robert Bradley

   Article

   Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

   Insert

   DUX4-3'UTR-intron2(-) (DUX4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouseCMV

   Available since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pRKB133_GI-DUX4-intron2(+)

   Plasmid

   #83967

   Purpose

   to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

   Depositor

   Robert Bradley

   Article

   Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

   Insert

   DUX4-3'UTR-intron2(+) (DUX4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouseCMV

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pSMOX-mNG::LacI-V3

   Plasmid

   #210224

   Purpose

   Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   mNeongreen Lac repressor fusion protein
   

   Use

   Trypanosoma brucei expression

   Tags

   Expression

   Mutation

   Promoter

   Pol II readthrough transcription after integration

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pILGFP97

   Plasmid

   #83551

   Purpose

   Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   CYC1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPMVAugw

    Plasmid

    #98300

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TEFM1EfmvaE>TEBS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-3xHAtag-SP-PTPRO

    Plasmid

    #191696

    Purpose

    For expression coding sequencing of PTPRO

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, 3xHAtag and full length coding sequence of PTPRO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. tdEos-VSVG-16

    Plasmid

    #57693

    Purpose

    Localization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    VSVG
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLV-EF1a-V5-Sec61B

    Plasmid

    #120244

    Purpose

    Expresses V5-tagged Sec61B in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Sec61B (Sec61b Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. RCAS (A) Wnt3a (CT#169)

    Plasmid

    #13976

    Depositor

    Cliff Tabin

    Article

    Kengaku et al Science. 1998 May 22. 280(5367):1274-7.

    Insert

    Wnt3a (Wnt3a Mouse)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-Nxph4-HA

    Plasmid

    #128102

    Purpose

    Express HA tagged Nxph4

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 2-Luc-Del-50

    Plasmid

    #21730

    Depositor

    Wafik El-Deiry

    Article

    Zeng et al Nat Genet. 1997 Jan . 15(1):78-82.

    Insert

    p21 promoter deletion mutant (CDKN1A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Identical to 2-Luc except that the 50-bp region b…

    Promoter

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-SP-CLSTN2-3xHAtag

    Plasmid

    #191693

    Purpose

    For expression coding sequencing of CLSTN2

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, full length coding sequence of CLSTN2 and 3xHAtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3×FLAG-CMV-14-LanCL2

    Plasmid

    #73391

    Purpose

    Expresses FLAG tagged LanCL2

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    LanCL2 (LANCL2 Human)
    

    Use

    Tags

    3x FLAG tagged

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MCV-Rep-

    Plasmid

    #32058

    Depositor

    Patrick Moore

    Article

    Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

    Insert

    MCV-Rep- genome
    

    Use

    Tags

    Expression

    Mutation

    Single cytosine to adenine (C/A) mutation at 2,07…

    Promoter

    none from vector. Insert contains native promoter…

    Available since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTYB3-human PUM1-HD MUT6-2 7-2 (N1043S-Q1047E-S1079N-E1083Q)-intein-CBD

    Plasmid

    #73300

    Purpose

    Expresses mutant human PUM1-HD

    Depositor

    Traci Hall

    Article

    Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

    Insert

    human PUM1 Pumilio homology domain (PUM1 Human)
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    N1043S-Q1047E-S1079N-E1083Q

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only