We narrowed to 32,635 results for: Eng

1. dpYPQ230-D156R-CBE4

   Plasmid

   #195369

   Purpose

   dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   hA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI
   

   Use

   CRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…

   Expression

   Plant

   Promoter

   ZmUBI1

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CCR5_gRNA_pX459

   Plasmid

   #192268

   Purpose

   gRNA expression for CRISPR Genome Editing of CCR5

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   CCR5 guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4/TO-CDC25A

   Plasmid

   #216511

   Purpose

   Expresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoter

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CDC25A (CDC25A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. prDNA spacer-LacOx120-20xMS2-Hyg

   Plasmid

   #210220

   Purpose

   Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   120xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-EGFP-NLS

   Plasmid

   #165441

   Purpose

   Expresses nuclear EGFP from CMV promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

   Insert

   EGFP-NLS
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHIV-iRFP720-E2A-Luc

   Plasmid

   #104587

   Purpose

   Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

   Depositor

   Antonius Plagge

   Article

   Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

   Insert

   iRFP720-E2A-Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   bi-cistronic

   Expression

   Mammalian

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pre_IV_CS10_1545_v1

   Plasmid

   #126861

   Purpose

   4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS10_1545_v1
   

   Use

   Synthetic Biology

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pre_IV_CS10_1033_v1

   Plasmid

   #126860

   Purpose

   4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS10_1033_v1
   

   Use

   Synthetic Biology

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3541 pMIG Bcl-XL

    Plasmid

    #8790

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9n(BB) (PX460)

    Plasmid

    #48873

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJH4783

    Plasmid

    #200785

    Purpose

    Pnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorin

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    snb-1 (snb-1 Nematode)
    

    Tags

    pHiuorin

    Expression

    Worm

    Promoter

    Pnpr-4

    Available Since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCAGGS_cAMPFIRE-M

    Plasmid

    #182284

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSFFV_sfCherry3V(1-10)

    Plasmid

    #117483

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3V(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3V(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    6 substitutions (K45R, G52D, L83W, T106A, K182R, …

    Promoter

    SFFV

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFW2500

    Plasmid

    #224213

    Purpose

    Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1 and recT
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9n(BB)-2A-Puro (PX462)

    Plasmid

    #48141

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pAAVS1-TetOn-dCas9-KRAB

    Plasmid

    #115545

    Purpose

    Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

    Depositor

    Rene Maehr

    Article

    Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

    Type

    Empty backbone

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSFFV-SpyTag-sfCherry2(11)-TagBFP

    Plasmid

    #117485

    Purpose

    Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    SpyTag002-GG-sfCherry2(11)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42229

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLS-CMV-mP-Rluc

    Plasmid

    #213507

    Purpose

    CMV-driven Renilla luciferase expression vector for luciferase lentivirus-based reporter assay

    Depositor

    Nadav Ahituv

    Article

    Deng et al Science. 2024 May 24;384(6698):eadh0559.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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