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pME-mKate-P2A-APEX2-NS

Plasmid

#67670

Purpose

Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-NS

Use

Gateway multisite middle entry clone

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_SynP_DIO_tAKARα _WPRE

Plasmid

#196675

Purpose

AAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).

Depositor

Haining Zhong

Publication

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

tAKARα

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-natCX

Plasmid

#73893

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

natCX

Expression

Yeast

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pFB2F

Plasmid

#195289

Purpose

Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

Depositor

Scott Lowe

Publication

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mScarlet

Expression

Mammalian

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFB2C

Plasmid

#195285

Purpose

Bicistronic HSV-TK and Hygromycin resistance for positive/negative selection

Depositor

Scott Lowe

Publication

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Hygro

Expression

Mammalian

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shSOD1

Plasmid

#102975

Purpose

Suppression of SOD1

Depositor

Richard Possemato

Publication

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shSOD1 (SOD1 Human)

Use

Lentiviral and RNAi

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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NRCoC

Plasmid

#74300

Purpose

Mammalian expression of full-length treacle fused to GFP

Depositor

Rita Shiang

Publication

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pERB219

Plasmid

#61501

Purpose

Const. Halotag-GFP-PACT + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Publication

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-PACT
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINVH

Plasmid

#26041

Depositor

Ray Owens

Publication

Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

Insert

u-LacZ-KHHHHHH

Tags

KHHHHHH and u-phosphatase secretion leader

Expression

Bacterial, Insect, and Mamm…

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 1.1kb promoter

Plasmid

#20125

Depositor

Warren Strober

Publication

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
pET28-His6-Ubc9

Plasmid

#133909

Purpose

Bacterial expression of His6-Ubc9

Depositor

Jeffrey Bode

Publication

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Cry2L348F(1-531)-mCh-BAXS184E

Plasmid

#117239

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Publication

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531.L348F-mCh-BAXS184E

Expression

Mammalian

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1354

Plasmid

#69490

Purpose

Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

Depositor

Jordan Ward

Publication

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope

Use

CRISPR; Template to generate cassettes for -media…

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKIV(1-313)

Plasmid

#45063

Purpose

Expression of constit active CaMKIV

Depositor

Richard Maurer

Publication

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Cam Kinase IV(1-313) (Camk4 Mouse)

Expression

Mammalian

Mutation

truncated at amino acid 313*

Promoter

RSV

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSCV-IRES-GFP-MD2-HA

Plasmid

#133065

Purpose

Retroviral expression of mouse MD2 tagged with HA

Depositor

Jonathan Kagan

Publication

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (Ly96 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

Plasmid

#140018

Purpose

Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Publication

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

EcoTyrRS

Tags

FLAG

Expression

Mammalian

Mutation

Y37L, D182S, F183M, L186A

Promoter

EF1

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NpuDnaEc-cGCaMP2

Plasmid

#34851

Depositor

Kevin Truong

Publication

Wong et al Cell Calcium. 2011 Nov 29.

Insert

NpuDnaEc-cGCaMP2

Expression

Bacterial and Mammalian

Promoter

CMV, T7 lac

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pET-UbcH10-DN

Plasmid

#8502

Depositor

Joan Ruderman

Publication

Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

Insert

UbcH10 (UBE2C Human)

Tags

His

Expression

Bacterial

Mutation

Dominant Negative, C114S

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pAAV-beta-arrestin2-HA-TEV219

Plasmid

#104845

Purpose

b2AR-SPARK protease component

Depositor

Alice Ting

Publication

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

beta-arrestin2-HA-TEV219

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSP-G2

Plasmid

#64737

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Publication

Partow et al Yeast. 2010 Nov;27(11):955-64. doi: 10.1

Type

Empty backbone

Expression

Yeast

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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