We narrowed to 8,228 results for: gnal

1. hGli3 NTOTAL in AINGpCAGGS

   Plasmid

   #121973

   Purpose

   medium Gli3 transcriptional repressor

   Depositor

   James Briscoe

   Article

   Ulloa et al Curr Biol. 2007 Mar 20;17(6):545-50. doi

   Insert

   hGli3 NTOTAL (GLI3 Human)
   

   Use

   Chick electroporation

   Tags

   5xmyc and Linker A IRES NLS GFP pCAGGS

   Expression

   Mammalian

   Mutation

   C terminus deletion from zinc finger domain

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GABPA_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176970

   Purpose

   Mammalian expression vector encoding GABPA and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   GABPA (GABPA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA8-Flag-LARS(1-976aa)

   Plasmid

   #139689

   Purpose

   Expresses N-teminal Flag tagged aa 1-976 of LARS1

   Depositor

   Jie Chen

   Article

   Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

   Insert

   LARS1 (LARS1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIP-CMV-mtagBFP2-2A-FLAG-cGAS E225A/D227A

   Plasmid

   #102604

   Purpose

   Lentivector to express Flag-tagged and catalytically-inactive cGAS and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Insert

   cGAS (CGAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   E225A-D227A

   Promoter

   CMV

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. InsRA-EBFP2

   Plasmid

   #79797

   Purpose

   Insulin receptor isoform A with a inter-domain EBFP2 tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   InsRA (INSR Human)
   

   Use

   Tags

   EBFP2 extracellular

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. anti-Notch1_E6-pBIOCAM5

   Plasmid

   #39344

   Depositor

   John McCafferty

   Article

   Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

   Insert

   scFv-Fc-fusion specific for Notch1 (clone B9) (Notch1, NOTCH1 Human, Mouse)
   

   Use

   Tags

   3xFLAG, 6xHis, and human Fc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. GNAS_pLENTI-CAG-IRES-GFP

   Plasmid

   #176991

   Purpose

   Mammalian lentiviral expression vector encoding GNAS

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   GNAS (GNAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P2-MDMX (23-111)

   Plasmid

   #62064

   Purpose

   Expression of human MDM4 (residues 23-111) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   MDM4 (MDM4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains residues 23-111

   Promoter

   tac

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-ALK3 K261R

   Plasmid

   #80875

   Purpose

   mammalian expression of ALK3 K261R

   Depositor

   Aristidis Moustakas

   Article

   Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

   Insert

   ALK3 (BMPR1A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K261R (Kinase inactive)

   Promoter

   CMV

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLNCX2-OptoTGFBRs

    Plasmid

    #118965

    Purpose

    Retroviral expression OptoTGFBRs for transient or stable constitutive expression

    Depositor

    Won Do Heo

    Article

    Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

    Insert

    OptoTGFBRs (Tgfb1, Tgfbr2 Mustard Weed, Mouse)
    

    Use

    Retroviral

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Hygro(+)-PSTCD-cGAS-delta DBD

    Plasmid

    #102606

    Purpose

    Mammalian expression of mutant cGAS with deletion of DNA binding domain fused to PSTCD tag

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (CGAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta K173-I220 and delta H390-C405

    Promoter

    CMV

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRepair-mScI-CTNNB1

    Plasmid

    #153431

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    mScarlet-i

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MBP-rPKM2 WT

    Plasmid

    #107158

    Purpose

    MBP-rPKM2 WT for production and purification of rat PKM2 WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMG059 MBP-CREB-His

    Plasmid

    #154076

    Purpose

    Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CREB1 (CREB1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5FRT/TO-Ataxin3-Strep-HA

    Plasmid

    #113496

    Purpose

    Expression of human Ataxin-3 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    Ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Flag-RNF168 delta RING

    Plasmid

    #133982

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of RING

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pPGK-T7/2-CD44v6 (-cyt)

    Plasmid

    #137817

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6 (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region, R296K (please see dep…

    Promoter

    PGK

    Available since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cry2 mCherry GRK2 ct in pcDNA3.1

    Plasmid

    #64212

    Purpose

    mCherry fused between Cry2 and GRK2ct to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    GRK2 ct (GRK2 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and cmv

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-GFP-IMP2-2_antisense-control

    Plasmid

    #42174

    Depositor

    Alexandra Kiemer

    Article

    Kessler et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1(+)-mCherry-p115(S240A)

    Plasmid

    #181997

    Purpose

    Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mCherry-p115(S240A) (ARHGEF1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Serine 240 changed to alanine in p115.

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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