We narrowed to 16,715 results for: sequence
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Plasmid#58903PurposeBacterial expression of bovine pancreatic ribonuclease ADepositorInsertRNase A (RNASE1 Bovine)
TagspelB sequenceExpressionBacterialMutationSee Depositor Comments belowPromoterT7Available SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI3
Plasmid#42527DepositorInsertWIPI3 (WDR45B Human)
TagsGST-TEVExpressionMammalianMutationgene sequence is codon optimized for expression i…Available SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
R57C10-Flp2
Plasmid#63172PurposeR57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusionDepositorInsertyeast wild type containing a Glycine at amino acid 5 of the protein sequence
ExpressionInsectAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-19:LAMP1-mEGFP
Plasmid#101782PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1DepositorInsertLAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianAvailable SinceNov. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMJ920
Plasmid#42234DepositorInsertCas9
UseCRISPRTagsGFP, HA epitope, and NLS (nuclear localization si…ExpressionMammalianMutationcodon-optimized synthetic DNA sequencePromoterCMVAvailable SinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLSL
Plasmid#51493PurposeT-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.DepositorTypeEmpty backboneUsePlant t-dna plasmidPromotervirion-sense LIR and 2x35SAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
gRNA Cloning Vector Bbs I ver. 2
Plasmid#85586PurposeAn empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRF2-IRESS-eGFP
Plasmid#19798DepositorInsertTRF2 (TERF2 Human)
ExpressionMammalianMutationWhen compared to GenBank reference sequence NP_00…PromoterCMV-FAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMK782 Septin6-GFP
Plasmid#38296DepositorInsertSeptin 6 (Septin6 Mouse)
TagseGFPExpressionMammalianMutation22 amino acid linker sequence (added by PCR)PromoterCMVAvailable SinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-deltaOVA
Plasmid#64595PurposeExpression of N-terminally truncated chicken ovalbumin as model antigen for study of MHC class I and class II presentationDepositorInsertOvalbumin
ExpressionMammalianMutationLacking first 49 aa (leader sequence for transloc…PromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-AcGFP-progerin
Plasmid#86947PurposeConstruct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.DepositorInsertAcGFP-progerin (LMNA Human)
UseAAVTagsAcGFPExpressionMammalianMutationprogerin sequence is LMNA gene with nucleotide C1…PromoterCMV and T7Available SinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_sgRNA
Plasmid#100554PurposeExpresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGATDepositorInsertAAVS1 sgRNA
PromoterU6Available SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
MKbpAII
Plasmid#91689PurposeMMTV-LTR promoter/enhancer with rabbit beta-globin intron and bovine growth hormone poly-A sequence to enhance transgene expressionDepositorInsertMMTV LTR cloned into KbpA vector
ExpressionMammalianPromoterMMTV LTRAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pREP
Plasmid#51491PurposepMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf virusDepositorInsertRep/RepA
UsePlant expression t-dnaMutationnonePromoter2x35SAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKT-iRFP-KAN
Plasmid#64687PurposepKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.DepositorInsertiRFP
ExpressionYeastMutationNucleotide sequence has been yeast-codon optimize…Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB1610_pRR-Puro
Plasmid#65853PurposePuromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9DepositorInsertsplit puromycin N-acetyltransferase coding sequence
UseCRISPR and TALENExpressionMammalianPromoterSV40Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
BTV
Plasmid#84771Purposebidirectional tetracycline-regulated viral reporter that measures the effects of 3' UTR sequences on mRNA and protein productionDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTR47m4
Plasmid#104068PurposeLuciferase Reporter for Formaldehyde SensingDepositorInsertsxluxAB
frmR
frm Promoter m4
UseLuciferase and Synthetic BiologyExpressionBacterialMutation23r and 25th nucleotides in sequence mutated from…Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT
Plasmid#19291DepositorInsertE-cadherin
UseLuciferaseExpressionMammalianMutationE-cad promoter sequences from -108 to +125 Ebo…Available SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDB4282
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only