We narrowed to 11,199 results for: AGA

1. pALS2-sfGFP WT

   Plasmid

   #197575

   Purpose

   Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP WT
   M. alvus Pyl-tRNA (6)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   araC and lpp

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAPM-miR30-IFIH1-ts3

   Plasmid

   #174261

   Purpose

   IFIH1 knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   IFIH1 shRNA (IFIH1 Human)
   

   Use

   Lentiviral and RNAi

   Promoter

   SFFV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-TRIM24

   Plasmid

   #65396

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   TRIM24 (TRIM24 Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDEST53-LRRK2-Y1699C

   Plasmid

   #25048

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tyrosine 1699 mutated to Cysteine

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

5. PIK3C2A gRNA (BRDN0001149181)

   Plasmid

   #76414

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3C2A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3C2A (PIK3C2A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. STARR-seq luciferase validation vector_ORI_SV40

   Plasmid

   #99309

   Purpose

   Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   SV40 enhancer
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Expression

   Mammalian

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA4-FLAG-Jhdm2a-H1122A

   Plasmid

   #38140

   Depositor

   Toshinobu Nakamura, Toru Nakano

   Article

   Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

   Insert

   Jhdm2a-H1122A (Kdm3a Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   protein starts at aa115; H1122A

   Promoter

   CMV

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PDI-QUAD-V5-pcDNA3.1

   Plasmid

   #153550

   Purpose

   PDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.

   Depositor

   Julie Atkin

   Article

   Parakh et al iScience. 2020 May 22;23(5):101097. doi:

   Insert

   Protein disulphide isomerase
   

   Tags

   V5 tag

   Mutation

   C53S, C56S, C397S, C400S

   Promoter

   pCMV

   Available Since

   Aug. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHCD1C

   Plasmid

   #102954

   Purpose

   gene cloning of human CD1C

   Depositor

   Gennaro De Libero, Lucia Mori

   Article

   Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

   Insert

   CD1C cDNA (CD1C Human)
   

   Use

   Cloning

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. UPB-esophagus (UPB5)

    Plasmid

    #180783

    Purpose

    Unique Projection Barcode (UPB5)-Esophagus for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    hM3Dq ( partial ) (CHRM3 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA4c/His-Xpress-HA-hBIG1(E793K)

    Plasmid

    #79433

    Purpose

    Expresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

    Insert

    BIG1 (ARFGEF1 Human)
    

    Tags

    HA, His6, and Xpress

    Expression

    Mammalian

    Mutation

    changed Glutamic acid 793 to Lysine

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pLT 653

    Plasmid

    #59996

    Purpose

    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

    Depositor

    Lisa Timmons

    Article

    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

    Inserts

    dhc-3 (dhc-3 Nematode)
    klp-16 (klp-16 Nematode)
    

    Expression

    Bacterial

    Mutation

    partial genomic

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV.hSynapsin1.OxLight-ctr

    Plasmid

    #169793

    Purpose

    Control non-binding fluorescent reporter for orexin neuropeptides

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

    Insert

    OxLight-ctr
    

    Use

    AAV

    Mutation

    E54K; T111A

    Promoter

    human Synapsin-1

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCRISPR v2-sgBAX-1

    Plasmid

    #210013

    Purpose

    knock out BAX in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Apoptosis regulator BAX (BAX Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lenti-EGFR-L858R-T790M-dual-epegRNA

    Plasmid

    #214100

    Purpose

    Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    EGFR L858R epegRNA/EGFR T790M epegRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-HA

    Plasmid

    #162100

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-StrepTagII-HA
    

    Use

    Lentiviral

    Tags

    VSVg-StrepTagII-HA

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LAMP-mChF-AIP

    Plasmid

    #61524

    Purpose

    Expression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIP

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Tags

    AIP and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-Foxo1-ADA 6KR

    Plasmid

    #17561

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1-ADA 6KR (Foxo1 Mouse)
    

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    K242, K245, K259, K262, K271 and K291 were replac…

    Promoter

    CMV

    Available Since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

19. STARR-seq luciferase INF enhancer vector_ORI_MX1

    Plasmid

    #99323

    Purpose

    Luciferase validation vector with MX1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    enhancer; chr21: 42791443 -42793515
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-HA

    Plasmid

    #162103

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-ProtC-HA
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-HA

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge