We narrowed to 7,766 results for: loxP
-
Plasmid#179451PurposeDonor vector to knock in Halotag C-terminal to human PER2 geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHaloTag (Halo7 Synthetic)
UseCRISPRTagsHis/FlagPromoterNoneAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB-hCRY1-SNAP-CD4-bla
Plasmid#179447PurposeDonor vector to knock in SNAPtag C-terminal to human CRY1 geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSnapTag
UseCRISPRTagsHis/FlagPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-Haunt-EF1a-DsRed
Plasmid#92162PurposeHaunt in trans overexpression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHaunt (Halr1 Mouse)
ExpressionMammalianPromoterCAGAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP1070-Ai211 (TITL-ChrimsonR-tdT-Kv2.1-ICF-IRES-tTA2)
Plasmid#181764Purposetargeting vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKv2.1-tagged ChrimsonR-tdTomato and tTA2
ExpressionMammalianAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -0
Plasmid#173889PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8-112–858_Flag2-Blast
Plasmid#175039PurposeMammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationExpression of MPP8 protein from amino acid positi…PromoterCAGAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8-1–729_Flag2-Blast
Plasmid#175040PurposeMammalian expression of mMPP8 (M1 to E729) with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationExpression of MPP8 protein from amino acid positi…PromoterCAGAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8-∆ARD_Flag2-Blast
Plasmid#175041PurposeMammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationExpression of MPP8 protein with deletion of the a…PromoterCAGAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode
Plasmid#170236PurposeBarcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -1
Plasmid#173890PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP108 Nanog-Venus-2a-mCherry
Plasmid#159743PurposeNanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNanog homeobox (Nanog Mouse)
UseMouse TargetingTagsVenus-2a-mCherryAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)
Plasmid#169194PurposeBaculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cellsDepositorInsert3xFlag-His6-PLpro (ORF1ab Synthetic, SARS-CoV-2)
Tags3xFlag-His6ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)
Plasmid#169164PurposeBaculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cellsDepositorInsertnsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
Tags6His-3xFlag (nsp10 C-terminus)ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)
Plasmid#169166PurposeBaculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cellsDepositorInsert3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)
Tags3xFlag-6HisExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)
Plasmid#169181PurposeBaculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cellsDepositorInsertnsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
TagsNo purification tag and nsp7-nsp8 fusion protein …ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-UBC9-sh3
Plasmid#168987PurposeExpresses RFP cDNA and UBC9 shRNA 3 in mammalian cellsDepositorAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-UBC9-sh1
Plasmid#168986PurposeExpresses RFP cDNA and UBC9 shRNA 1 in mammalian cellsDepositorAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP
Plasmid#162336PurposeLentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralExpressionMammalianPromoterhU6; mU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::T48-Baz::mCherry
Plasmid#163927PurposeExpression of GrabFP-A-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Nrv1::tagBFP
Plasmid#163925PurposeExpression of GrabFP-B-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
UseCre/LoxTagstagBFPExpressionInsectAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only