We narrowed to 5,949 results for: pcas

1. ABE8e(TadA-8e K20A R21A)

   Plasmid

   #138496

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e K20A R21A)-nSpCas9
   

   Expression

   Mammalian

   Mutation

   TadA-8e K20A R21A

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. VRER variant

   Plasmid

   #138561

   Purpose

   Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRER
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPQD1CB0202

   Plasmid

   #185626

   Purpose

   MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-sgRNA_Dicer_3

   Plasmid

   #68809

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Px458-Cas9n-Trp63-Exon4-2sgRNA

   Plasmid

   #88849

   Purpose

   CRISPR KO of Trp63

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp63 (Trp63 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

6. AtS/MAR10 insulator sequence (GB3458)

   Plasmid

   #160638

   Purpose

   pEGB3a1 AtS/MAR10

   Depositor

   Diego Orzaez

   Article

   Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

   Insert

   AtS/MAR10 insulator sequence
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

   Plasmid

   #159658

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Promoter

   Ef1a

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

   Plasmid

   #159657

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Promoter

   Ef1a

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-sgRNA_Dicer_2

   Plasmid

   #68808

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

    Plasmid

    #160610

    Purpose

    Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:AsCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAT-9222

    Plasmid

    #124222

    Purpose

    Cloning plasmid for EGxFP assay with self-targeting gRNA-s

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA between EGFP halves
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 Puro shRNA INPP5E KD1

    Plasmid

    #162004

    Purpose

    INPP5E shRNA

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    INPP5E (INPP5E Canis Lupus)
    

    Use

    Lentiviral

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. hs-lambda btl

    Plasmid

    #17581

    Depositor

    Denise Montell

    Article

    Lee et al Genes Dev. 1996 Nov 15. 10(22):2912-21.

    Insert

    lambda-btl (btl Fly)
    

    Expression

    Insect

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

14. Djun - sense

    Plasmid

    #38337

    Depositor

    Norbert Perrimon

    Article

    Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

    Insert

    djun (Jra Fly)
    

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

15. AAEL007584-Cas9

    Plasmid

    #100706

    Purpose

    The promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL007584-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL007584 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT45_GalL_nCDA1-BE3

    Plasmid

    #145038

    Purpose

    Expresses nCDA1-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

    Plasmid

    #160635

    Purpose

    TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRII-Topo U6-SpAi9L-U6SpDMDL

    Plasmid

    #78608

    Purpose

    Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9L-U6SpDMDL
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pCRII-Topo U6-SpAi9R-U6-SpDMDR

    Plasmid

    #78609

    Purpose

    Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9R-U6-SpDMDR
    

    Use

    Unspecified

    Promoter

    U6

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pBW1997_pExpr-hU6-CRISPR-DECODER

    Plasmid

    #87549

    Purpose

    Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    gRNAs targeting NGN2, MIAT, ACTC1, TTN
    

    Use

    CRISPR, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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