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pT7-EGFP-C1-HsGIGYF2_1-180-YYMLtoAAAA_AE

Plasmid

#148580

Purpose

Mammalian Expression of HsGIGYF2_1-180-YYMLtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-YYMLtoAAAA (GIGYF2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-HsGIGYF2_1-180-LFItoDDD_AE

Plasmid

#148582

Purpose

Mammalian Expression of HsGIGYF2_1-180-LFItoDDD

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-LFItoDDD (GIGYF2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EHP-I85AM101A_AE

Plasmid

#148583

Purpose

Mammalian Expression of Hs4EHP-I85AM101A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP-I85AM101A (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EHP-I85AW95AM101A_AE

Plasmid

#148584

Purpose

Mammalian Expression of Hs4EHP-I85AW95AM101A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP-I85AW95AM101A (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EHP-R103LE149L_AE

Plasmid

#148585

Purpose

Mammalian Expression of Hs4EHP-R103LE149L

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP-R103LE149L (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EHP-W95AR103LE149L_AE

Plasmid

#148587

Purpose

Mammalian Expression of Hs4EHP-W95AR103LE149L

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP-W95AR103LE149L (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G1_615-654-L640AV646A_AD

Plasmid

#148519

Purpose

Mammalian Expression of HseIF4G1_615-654-L640AV646A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-L640AV646A (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD

Plasmid

#148523

Purpose

Mammalian Expression of HseIF4G1_615-654-LIVVtoAAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-LIVVtoAAAA (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-Y619AL624AL625A_AD

Plasmid

#148524

Purpose

Mammalian Expression of HseIF4G1_615-654-Y619AL624AL625A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-Y619AL624AL625A (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-VP16

Plasmid

#183927

Purpose

Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-VP16 (CRY2, UL48 Synthetic, Mustard Weed)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-HseIF4E_AF

Plasmid

#148669

Purpose

Bacterial Expression of HseIF4E

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4E (EIF4E Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence of i…

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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SYKA-c022

Plasmid

#175490

Purpose

Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.

Depositor

Opher Gileadi

Article

Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

Insert

SYK (SYK Human)

Tags

AviTag and His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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p35S_EVDdi

Plasmid

#167120

Purpose

Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) lacking the intron under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE (EVD) (AT5G17125 Mustard Weed)

Expression

Plant

Mutation

Deleted intron from full length element

Promoter

CaMV 35S

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-HaloSFPQY527A

Plasmid

#166949

Purpose

Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

Halo

Mutation

SFPQ-Y527A

Promoter

eF1a

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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UbC-Halo-SFPQY527A

Plasmid

#166947

Purpose

Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

Halo

Mutation

SFPQ-Y527A

Promoter

UbC

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_Donor/Reporter-COL4A3

Plasmid

#130279

Purpose

COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

mCherry-eGFP

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_COL4A3_self-cleavingCas9

Plasmid

#130281

Purpose

Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

spCas9

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_COL4A5_self-cleavingCas9

Plasmid

#130282

Purpose

Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

spCas9

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-SOCS1-Y80D

Plasmid

#129516

Purpose

Retroviral vector for the expression of human SOCS1 with a Y80D mutation and a FLAG tag in N-terminal

Depositor

Gerardo Ferbeyre

Article

Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

Insert

SOCS1 (SOCS1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 80 to Aspartic acid

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-SOCS1-Y80F

Plasmid

#129517

Purpose

Retroviral vector for the expression of human SOCS1 with a Y80F mutation and a FLAG tag in N-terminal

Depositor

Gerardo Ferbeyre

Article

Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

Insert

SOCS1 (SOCS1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 80 to Phenylalanine

Promoter

cmv

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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