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Plasmid#202125PurposeLevel 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertFld promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI site for…PromoterAvailable sinceAug. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-54730
Plasmid#202109PurposeLevel 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert54730 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI site for…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-51183
Plasmid#202108PurposeLevel 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert51183 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI sites fo…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-GEF
Plasmid#202107PurposeLevel 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertGEF promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI site for…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-47740
Plasmid#202106PurposeLevel 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert47740 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI site for…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-BCA
Plasmid#202105PurposeLevel 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertBCA promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI sites fo…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-epyC
Plasmid#202104PurposeLevel 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertepyC promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI sites fo…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST_DHN_ShDHN (pBS0797)
Plasmid#185260PurposeFor the mammalian expression of the tomato plant protein DHN_ShDHN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertDHN_ShDHN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)
Plasmid#159664PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)
Plasmid#159663PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)
Plasmid#159662PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)
Plasmid#159656PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_FH-AGO2-WT
Plasmid#92006PurposeExpresses FLAG-HA-AGO2 (WT)DepositorInsertAGO2 (AGO2 Human)
UseTagsFLAG-HAExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-Flag-c-Myc
Plasmid#102625PurposeExpresses c-Myc in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-T2TP
Plasmid#114725Purposeexpression in mammalian cellsDepositorInsertTol2 transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRES2_EGFP_KCNQ1
Plasmid#173161PurposeFor mammalian expression of KCNQ1 and creating variantsDepositorInsertKCNQ1 (KCNQ1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH
Plasmid#184463PurposepENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH express N-flag tag STAT5bCA carrying a point mutation at base 1963 (A to C), which changes Asn642 to His, conferring constitutive activity (CA) to STAT5b.DepositorInsertN-Flag-mSTAT5bCA (Stat5b Mouse)
UseTagsFLAG TagExpressionMammalianMutationPoint mutation at base 1963 (A to C), which chang…PromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-SOCS1
Plasmid#129514PurposeRetroviral vector for the expression of human SOCS1 with a FLAG tag in N-terminalDepositorInsertSOCS1 (SOCS1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-BCR-ABL-p190-FLAG
Plasmid#205620PurposeExpression of BCR-ABL oncogene in mammalian cellsDepositorInsertBCR-ABL oncogene, p190 isoform b3a2
UseTagsFLAGExpressionMammalianMutationT117M- Please see depositor commentsPromoterCMVAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab27B
Plasmid#89447PurposeExpression of GFP-tagged human Rab27B in mammalian cellsDepositorInserthuman Rab27B (RAB27B Human)
UseTagseGFPExpressionMammalianMutationwild typePromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cre-p2a-tdTomato
Plasmid#176837PurposeExpress Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expressionDepositorInsertCre-P2A-tdTomato
UseTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
His Puc (aa 1-393)
Plasmid#52295PurposeBacterial expression of His tagged Drosophila Puc fragmentDepositorInsertPuc (puc Fly)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6XHis-SUMO-SIRT2
Plasmid#102622PurposeExpresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purificationDepositorInsertSIRT2 (SIRT2 Human)
UseTags6XHis-SUMOExpressionBacterialMutationDeleted amino acids 1-37 and 357-389PromoterAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9
Plasmid#210703PurposeThis plasmid express EFS promoter driven human codon optimized SpdCas9DepositorInsertS. Pyogenes dCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A/H840APromoterEF1a core promoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCherryGFP-CoV2-Inframe-stop
Plasmid#177620PurposeDual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).DepositorInsertSARS-CoV-2 FSE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-HA-GATA3 FL
Plasmid#89835PurposeExpress GATA3 in mammalian cellsDepositorInsertGata3 (Gata3 Mouse)
UseRetroviralTagsHAExpressionMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Neo-EF1A>Tet3G
Plasmid#184379PurposeThe Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)DepositorInsertTet3G
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-delStem1
Plasmid#177616PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutation3-nucleotide (UAC) deletion in Stem 1PromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-tTA
Plasmid#127091PurposeAn AAV genome with Cre-dependent expression of tTA from the CAG promoterDepositorInserttTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterCAGAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-delSS
Plasmid#177615PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. Slippery sequence (SS) deleted (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutationSlippery sequence deletedPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MO70-N-Flag-hCRACR2A-isoform-a
Plasmid#79592PurposeTransient and retroviral expressionDepositorInsertCRACR2A-isoform-a (CRACR2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGG-9.47_VP1,3 #2515
Plasmid#74237PurposeModified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47DepositorInsertsAAV2 Rep
AAV9.47 VP1
AAV9.47 VP3
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330 LC-FKBP-Cas9
Plasmid#162724PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-hCRACR2A-isoform-a
Plasmid#79593PurposeTransient expressionDepositorInsertCRACR2A-isoform-a (CRACR2A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-Nix
Plasmid#100770PurposeLentiviral expression of shRNA targeting NixDepositorInsertLenti-sh-Nix
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS-dsarm(E)
Plasmid#187870PurposeGal4/UAS expression of dSarm(isoE)DepositorInsertdSarm isoform E (Sarm Fly)
UseTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1
Plasmid#176839PurposeTo recruit SAM complex to human CYP3A4 proximal enhancer.DepositorInsertHuman CYP3A4 proximal enhancer sgRNA for activation
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 NL-FKBP-Cas9
Plasmid#162723PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BTB+BACK)
Plasmid#169723PurposeMammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)DepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationL186D, L190D, L193D, I217K, Y353EPromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_LN-HA-AGO2-WT
Plasmid#92009PurposeExpresses Lambda-N-HA-AGO2 (WT)DepositorInsertAGO2 (AGO2 Human)
UseTagsLambda-N-HAExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only