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MAPK3_HUMAN_D0

Plasmid

#79702

Purpose

This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MAPK3 (MAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MAPK3 gRNA (BRDN0001147876)

Plasmid

#75967

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK3 (MAPK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK3 gRNA (BRDN0001147667)

Plasmid

#75968

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK3 (MAPK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK3 gRNA (BRDN0001149014)

Plasmid

#75966

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK3 (MAPK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK3

Plasmid

#23509

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK3 (MAPK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_MAPK3_WT

Plasmid

#82313

Purpose

Gateway Donor vector containing MAPK3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK3 (MAPK3 Human)

Use

Gateway entry vector

Mutation

L180X; p.135_136delSQ-insRPAAE; p.138_379del

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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EPHB2 gRNA (BRDN0001146273)

Plasmid

#77545

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB2 (EPHB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB2 gRNA (BRDN0001147469)

Plasmid

#77546

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB2 (EPHB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB2 gRNA (BRDN0001147792)

Plasmid

#77547

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB2 (EPHB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB2 gRNA (BRDN0001147198)

Plasmid

#77548

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB2 (EPHB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB2_HUMAN_D0

Plasmid

#79697

Purpose

This plasmid encodes the kinase domain of EPHB2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB2 (EPHB2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MAPK12 gRNA (BRDN0001146907)

Plasmid

#77485

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK12 (MAPK12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK12 gRNA (BRDN0001148543)

Plasmid

#77486

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK12 (MAPK12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK12 gRNA (BRDN0001147863)

Plasmid

#77487

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK12 (MAPK12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK4 gRNA (BRDN0001148116)

Plasmid

#77220

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK4 (MAPK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK4 gRNA (BRDN0001145383)

Plasmid

#77221

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK4 (MAPK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK4 gRNA (BRDN0001145865)

Plasmid

#77222

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK4 (MAPK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK4 gRNA (BRDN0001147141)

Plasmid

#77223

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK4 (MAPK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-N MCV ER

Plasmid

#37860

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

MCV ER (MCPyV_gp2, MCPyV_gp1 Human Merkel Cell Virus)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
PB-TAC-ERP2-(N-HA)KRAS_G12D

Plasmid

#201254

Purpose

Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

Depositor

Alexander Kleger

Article

Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

Insert

KRAS (KRAS Human)

Use

Piggybac transposon

Tags

HA-Tag

Expression

Mammalian

Mutation

changed Gly (G) 12 to Asp (D)

Promoter

TRE

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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