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Plasmid#222722PurposesfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)DepositorTypeEmpty backboneUseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only
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Arp2/3_pBIG2abcd
Plasmid#173683PurposeInsect expression of the 7-subunit Human Arp2/3 complexDepositorInsertsArp2
Arp3
ArpC2
ArpC3-FLAG
ArpC4
ArpC5
ArpC1
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP30_Encap
Plasmid#86406PurposeExpresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 30aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP15_Encap
Plasmid#86407PurposeExpresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 15aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-Pds1(434)
Plasmid#39847DepositorInsertPds1 (PTTG1 Human)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLPC flag-mRAP1 I312R
Plasmid#73138PurposeExpression of Flag-tagged mRAP1 with single point mutatoin I312RDepositorInsertRap1 (Terf2ip Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-sh-mFAK C
Plasmid#37015DepositorInsertsh-mFAK C (Ptk2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceMay 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-sh-mFAK B
Plasmid#37018DepositorInsertsh-mFAK B (Ptk2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceMay 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPD_Psra-6::ChR2(H134R)::mCherry
Plasmid#117419PurposeFor selectively expressing ChR2(H134R) in ASH sensory neurons under the expression of a Psra-6 promoterDepositorInsertchannel rhodopsin-2 with H134R mutation
UseTagsExpressionWormMutationH134RPromotersra-6Available sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH (Merlin)
Plasmid#136448PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-lengthDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pANY6
Plasmid#112198PurposeThis plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.DepositorTypeEmpty backboneUseTagsELP-tagExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-H2B-pr-mEosFP
Plasmid#141101PurposeH2B fused to pr-mEosFP for chromatin visualization and primed conversionDepositorInsertHistone 2B
UseTagspr-mEosFPExpressionMammalianMutationPromotersimian CMV IE94Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET14_GFP5_Encap
Plasmid#86408PurposeExpresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 5aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD_Pinx-1::ChR2(H134R)::mCherry
Plasmid#117420PurposeFor selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoterDepositorInsertchannel rhodopsin-2 with H134R mutation
UseTagsExpressionWormMutationH134RPromoterinx-1Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 (Delta Pest1)
Plasmid#78096Purposemammalian expression of GADD34 Delta Pest1DepositorInsertGADD34 (PPP1R15A Human)
UseTagsFLAGExpressionMammalianMutationdeletion of PEST1PromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-3nitroY-A7-RS
Plasmid#141173PurposeExpression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acidsDepositorInsertA7 3nY tRNA synthetase
UseLentiviralTagsExpressionMammalianMutationP186T L274R N311G C313NPromoterCMVAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pANY3
Plasmid#112197PurposeThis plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.DepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-PCNA-19-NLS-4
Plasmid#54222PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertPCNA-19 NLS (PCNA Human)
UseTagsSV40NLS and mEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac-LacI-LAP
Plasmid#114034PurposeProduction of baculovirus with LacI-LAPDepositorInsertLacI-LAP
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only