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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,530 results for: Kap

Showing: 521 - 540 of 2530 results

  1. pJK576

    Plasmid
    #72527
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein associated with purSyQL cluster (AaOrfY)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Gene synthesis
    Tags
    Expression
    Mutation
    synthetic gene
    Promoter
    n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72527-plasmid-map-sequence-id-132615

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  2. pJK602

    Plasmid
    #71607
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe mutant (AaPurE1-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tyrosine-154 …
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71607-plasmid-map-sequence-id-131684

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  3. pJK603

    Plasmid
    #71608
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe mutant (AaPurE1fww-Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71608-plasmid-map-sequence-id-131687

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  4. pJK663

    Plasmid
    #71612
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tyrosine-154 to phenylalanine, tryptophan…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71612-plasmid-map-sequence-id-131690

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  5. pJK665

    Plasmid
    #71614
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp97>Tyr mutant (AaPurE1fyw-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71614-plasmid-map-sequence-id-131692

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  6. pJK510

    Plasmid
    #71600
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp165>Phe mutant (AaPurE1wwf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-16…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71600-plasmid-map-sequence-id-131674

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  7. pCVD315

    Plasmid
    #63737
    Purpose
    Derivative of pKO-1 carrying the multiple cloning region from M13mp19RF
    Depositor
    James Kaper
    Article
    Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pRDA_867

    Plasmid
    #231109
    Purpose
    A>G base editor
    Depositor
    John Doench, David Root
    Insert
    ABE8e-nCas9-SpG-HA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D10A
    Promoter
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231109-plasmid-map-sequence-id-458425

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  10. pRDB_270

    Plasmid
    #231111
    Purpose
    C>T base editor
    Depositor
    John Doench, David Root
    Insert
    TadA-CDd-nCas9-SpG-UGI-HA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D10A
    Promoter
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    231111-plasmid-map-sequence-id-458638

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  19. pJK276

    Plasmid
    #73366
    Purpose
    Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)
    Depositor
    T. J. Kappock
    Insert
    plastid omega-3 fatty acid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    Mutation I231F. Also insert is missing residues 1…
    Promoter
    GAL1
    Available Since
    May 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    73366-plasmid-map-sequence-id-135095

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  20. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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Showing: 521 - 540 of 2530 results