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pJC2745 - hPGK-EGFP-ZMAT2(C-term)-IRES2-mCherry-EF1A-Puro

Plasmid

#250276

Purpose

Ratiometric protein stability reporter for measuring C-terminal degron activity.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

EGFP-ZMAT2(C-term)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2742 - hPGK-EGFP-SUGT1(C-term)-IRES2-mCherry-EF1A-Puro

Plasmid

#250273

Purpose

Ratiometric protein stability reporter for measuring C-terminal degron activity.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

EGFP-SUGT1(C-term)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2743 - hPGK-EGFP-MTFR1L(C-term)-IRES2-mCherry-EF1A-Puro

Plasmid

#250274

Purpose

Ratiometric protein stability reporter for measuring C-terminal degron activity.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

EGFP-MTFR1L(C-term)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2744 - hPGK-EGFP-GOPC(C-term)-IRES2-mCherry-EF1A-Puro

Plasmid

#250275

Purpose

Ratiometric protein stability reporter for measuring C-terminal degron activity.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

EGFP-GOPC(C-term)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2741 - hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro

Plasmid

#250272

Purpose

Ratiometric protein stability reporter for measuring C-terminal degron activity.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

EGFP-CycD1(C-term)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-Nb127D01-mCherry-KDEL

Plasmid

#171575

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-NbVHH05-mCherry-KDEL

Plasmid

#171574

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET-26b-Nb127D01-ALFA-His

Plasmid

#171568

Purpose

Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-ALFA-His (CXCR2 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-HGP-v3-NbVHH05-hIgG

Plasmid

#171565

Purpose

Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)

Tags

BiP signal peptide and hIgG1-hinge-N297A-TEV site…

Expression

Insect

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJC2739 - pHR-EF1A-HA-FBXO31-IRES-mTagBFP

Plasmid

#250270

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31)

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2728 - pLenti-X1-EF1A-HA-FBXO31(D334N)-PGK-Neo

Plasmid

#250260

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(D334N)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

D334N

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2723 - pLenti-X1-EF1A-HA-FBXO31-PGK-Neo

Plasmid

#250255

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC2740 - pHR-EF1A-HA-FBXO31(D334N)-IRES-mTagBFP

Plasmid

#250271

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(D334N))

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

D334N

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC2726 - pLenti-X1-EF1A-HA-FBXO31(I337D)-PGK-Neo

Plasmid

#250258

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(I337D)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

I337D

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC2727 - pLenti-X1-EF1A-HA-FBXO31(T343V)-PGK-Neo

Plasmid

#250259

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(T343V)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

T343V

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC2731 - pHR-EF1A-HA-FBXO31-IRES-GFP

Plasmid

#250263

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC2734 - pHR-EF1A-HA-FBXO31(D334N)-IRES-GFP

Plasmid

#250266

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(D334N)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

D334N

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC2732 - pHR-EF1A-HA-FBXO31(∆F-box)-IRES-GFP

Plasmid

#250264

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

∆F-box

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2737 - pHR-EF1A-DD-3xFLAG-FBXO31-IRES-GFP

Plasmid

#250268

Purpose

Inducible expression of FBXO31 with the shield degron system.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

DD-3xFLAG

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC2724 - pLenti-X1-EF1A-HA-FBXO31(∆F-box)-PGK-Neo

Plasmid

#250256

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

∆F-box

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge