We narrowed to 752 results for: Numb

1. pGDP1 vph

   Plasmid

   #112893

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   vph
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. hPlekhm1_pIRES puro Glue

   Plasmid

   #73453

   Purpose

   Express human Plekhm1 in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   CBP tag, HA tag, and strep tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGDP3 vatD

   Plasmid

   #112885

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   vatD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGDP3 aph(9)-Ia

   Plasmid

   #112882

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   aph(9)-Ia
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-CRISPR-Puro-sgRPL3-62

   Plasmid

   #208647

   Purpose

   pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

   Depositor

   Quanfu Ma

   Insert

   sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-CRISPR-Puro-sgRPL3-56

   Plasmid

   #208979

   Purpose

   pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

   Depositor

   Quanfu Ma

   Insert

   sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB6-Id1-T2A-sfGFP-iCre-ERT2-FNF-TK

   Plasmid

   #139279

   Purpose

   B6J Id1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   Id1 (Id1 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   C-terminal fusion of T2A-sfGFP-iCre-ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGDP1 uvrA

   Plasmid

   #112892

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   uvrA-like protein
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSCV-HA-shift-IRES-GFP(KaganE21)

   Plasmid

   #52004

   Purpose

   Expresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP marker

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS-Shift
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   A 2 nucleotide insertion was introduced between t…

   Promoter

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pTH729-minRLuc

    Plasmid

    #38225

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    Renilla Luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Every codon of the Renilla coding sequence was ex…

    Promoter

    TDH3 (=GPD)

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGDP1 NDM-1

    Plasmid

    #112883

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    NDM-1 (blaNDM-1 Klebsiella pneumoniae)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAC-ZEAXipi

    Plasmid

    #53287

    Purpose

    Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p5154

    Plasmid

    #175390

    Purpose

    High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)

    Depositor

    Michael Wiles

    Article

    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting; Cloning vector, to be used to de…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAC-LYC

    Plasmid

    #53270

    Purpose

    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

    Insert

    crtE, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC-CANTHipi

    Plasmid

    #53301

    Purpose

    Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtO
    

    Use

    Low copy number bacterial cloning vector

    Tags

    6His

    Expression

    Mutation

    Promoter

    Trc

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFR-mApple

    Plasmid

    #203773

    Purpose

    Number and brightness analysis of EGFR oligomerization

    Depositor

    Thorsten Wohland

    Article

    Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFR-PATagRFP

    Plasmid

    #31950

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

    Insert

    PATagRFP
    

    Use

    Tags

    EGF receptor (EGFR)

    Expression

    Mammalian

    Mutation

    R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

    Promoter

    CMV

    Available since

    Oct. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-VSVG

    Plasmid

    #11912

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F64L, S65T, A206K relative the wild type GFP sequ…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EGFR-mEGFP

    Plasmid

    #203774

    Purpose

    Number and brightness analysis of EGFR oligomerization

    Depositor

    Thorsten Wohland

    Article

    Gupta et al J Lipid Res. 2022 Jun;63(6):100220. doi:

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC-EHER

    Plasmid

    #53262

    Purpose

    Contains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

    Insert

    crtE, idi, crtX, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge