Addgene

We narrowed to 1,222 results for: TIR

Showing: 521 - 540 of 1222 results

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pHLP710

Plasmid

#203740

Purpose

Integration of OsTIR1-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Expression

Mutation

Promoter

ACT1 (C. albicans)

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEmCherry2

Plasmid

#118413

Purpose

Red fluorescent reporter for assessing CRISPR gRNA activity

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

mCherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Cag.Luc.puro

Plasmid

#74409

Purpose

Constitutive Luciferase expression in mammalian cells along with puromycin selection

Depositor

Georgios Stathopoulos

Article

Giannou et al EMBO Mol Med. 2017 May;9(5):672-686. doi

Insert

Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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Cdk6

Plasmid

#1866

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Use

Tags

Expression

Mammalian

Mutation

D224Y

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pAID-CMV linearizing in pX330

Plasmid

#140609

Purpose

Crispr/Cas9 plasmid for linearization of pAID-CMV

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

sgRNA for pAID-EF1a plasmid digestion

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+ Lifeact-mCherry

Plasmid

#185408

Purpose

Red fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

lifeact

Use

In vitro synthesis of mrna

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jGCaMP8f

Plasmid

#192615

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-RiboL1-jGCaMP7c

Plasmid

#192619

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RiboL1) jGCaMP7c.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP7c

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHLP712

Plasmid

#203741

Purpose

Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Expression

Mutation

Codon 74 changed from Phe to Ala (F74A)

Promoter

ACT1 (C. albicans)

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RiboL1-jGCaMP8s

Plasmid

#192611

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jRex-GECO

Plasmid

#172714

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jRex-GECO.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jRex-GECO

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-jRex-GECO

Plasmid

#169259

Purpose

AAV transfer plasmid for neuronal expression of jRex-GECO.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

JRex-GECO

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RiboL1-jGCaMP8f

Plasmid

#192612

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Cdk6-DN-HA

Plasmid

#1869

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-RiboL1-jGCaMP8m

Plasmid

#192613

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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3269 pBS Bcl-2

Plasmid

#8764

Depositor

Stanley Korsmeyer

Article

Silverman et al Proc Natl Acad Sci U S A 1990 Dec;87(24)

Insert

Bcl-2 (Bcl2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
Cdk6-DN

Plasmid

#1867

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Use

Tags

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pAP007

Plasmid

#206809

Purpose

pET-6xHis-MBP-TEV-BabTIR-APAZ/BabAgo - Heterologous BabSPARTA expression

Depositor

Daan Swarts

Article

Finocchio et al Nucleic Acids Res. 2024 Feb 28;52(4):201

Insert

BabSPARTA operon (6xHis-MBP-BabTIR-APAZ and BabAgo)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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hs-FL-Kek1-myc2

Plasmid

#41170

Depositor

Norbert Perrimon

Article

Ghiglione et al Cell. 1999 Mar 19;96(6):847-56.

Insert

Kek1 (kek1 Fly)

Use

Tags

2X myc

Expression

Insect

Mutation

Promoter

Hsp70

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
6691 Bicistronic_ires_puro

Plasmid

#64335

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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