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pTJK642

Plasmid

#138013

Purpose

SIX1 CRISPRi

Depositor

Tami Kingsbury

Insert

SIX1 sgRNA13 (SIX1 Human)

Use

CRISPR and Lentiviral

Mutation

None

Promoter

U6

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_SAFBgRNA9

Plasmid

#196108

Purpose

Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196107

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVR8.74

Plasmid

#22036

Purpose

2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

Depositor

Didier Trono

Insert

gag pol tat rev

Expression

Mammalian

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pLentiV2-dCas9-VP64

Plasmid

#141104

Purpose

Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

Depositor

Igor Ulitsky

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a- gfp-2a-puro

Plasmid

#129443

Purpose

expression of GFP and Puro R

Depositor

Oskar Laur

Insert

gfp-puro

Use

Lentiviral

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSOX11.1.0-gDNA

Plasmid

#176378

Purpose

Expresses spCas9 and gRNA sequence targeting SOX11

Depositor

Barbara Stranger, Kevin White

Insert

SOX11 gRNA

Use

CRISPR

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hSYN-TdTomato

Plasmid

#193019

Purpose

AAV vector plasmid expressing TdTomato under the human synapsin (hSYN) promoter

Depositor

Bart Nieuwenhuis

Insert

TdTomato

Use

AAV

Expression

Mammalian

Promoter

hSYN

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW-H2B-mCherry

Plasmid

#132333

Purpose

Third generation lentiviral vector for hUbC-driven H2B-mCherry expression

Depositor

Bjoern Schwer

Insert

H2B-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

July 18, 2020

Availability

Academic Institutions and Nonprofits only

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lis-1::L4440

Plasmid

#11336

Depositor

Guy Caldwell

Insert

lis-1 (lis-1 Nematode)

Use

RNAi

Expression

Worm

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pBACH2.2.0-gDNA

Plasmid

#176375

Purpose

Expresses spCas9 and gRNA sequence targeting BACH2

Depositor

Barbara Stranger, Kevin White

Insert

BACH2 gRNA

Use

CRISPR

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKG333

Plasmid

#69751

Purpose

Retroviral expression of Histone H3

Depositor

Iain Cheeseman

Insert

Histone H3

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Mutation

e.coli optimized sequence

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-BNIP3-FL

Plasmid

#100771

Purpose

Lentiviral expression of shRNA targeting BNIP3

Depositor

Joseph Gordon

Insert

Lenti-sh-BNIP3-FL

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLV CMV FST-HA

Plasmid

#175168

Purpose

Lentiviral expression of human FST-HA

Depositor

Larry Gerace

Insert

FST

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RP1B-PP1gamma

Plasmid

#51770

Purpose

Expresses PP1 gamma residues 7-323

Depositor

Wolfgang Peti

Insert

pp1 gamma

Tags

His, TEV protease site, and Thio-6

Expression

Bacterial

Mutation

Contains PP1 gamma residues 7-323

Promoter

T7-lac

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
pSOX2.1.0-gDNA

Plasmid

#176382

Purpose

Expresses spCas9 and gRNA sequence targeting SOX2

Depositor

Barbara Stranger, Kevin White

Insert

SOX2 gRNA

Use

CRISPR

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Peroxisomes-FAST

Plasmid

#216343

Purpose

For testing localization of pFAST protein to peroxisomes

Depositor

Alison Tebo

Insert

pFAST_SKL

Tags

myc for FAST protein

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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RP1B-PP1beta

Plasmid

#51769

Purpose

Expresses PP1 beta residues 6-327

Depositor

Wolfgang Peti

Insert

pp1 beta

Tags

His, TEV protease site, and Thio-6

Expression

Bacterial

Mutation

Contains PP1 beta residues 6-327

Promoter

T7-lac

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
nud-1::L4440

Plasmid

#11337

Depositor

Guy Caldwell

Insert

nud-1 (nud-1 Nematode)

Use

RNAi

Expression

Worm

Available Since

July 12, 2006

Availability

Academic Institutions and Nonprofits only
pKG422

Plasmid

#69752

Purpose

Retroviral expression of spc24

Depositor

Iain Cheeseman

Insert

spc24 (SPC24 Human)

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pNDMS291

Plasmid

#248098

Purpose

sfCFP fluorescent reporter for synthetic Rhizobium nifH promoter

Depositor

Barney Geddes

Insert

sfCFP

Expression

Bacterial

Promoter

Rlv_PsnifH

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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