We narrowed to 537 results for: gcg.2

1. lentiCRISPR v2-sgHSPC300-1

   Plasmid

   #210141

   Purpose

   knock out HSPC300 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Protein BRICK1 (BRK1 Human)
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

   Plasmid

   #165086

   Purpose

   gRNA 2 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGMC00005

   Plasmid

   #166722

   Purpose

   sgRNA against human ASNS

   Depositor

   Raj Chari

   Article

   Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

   Insert

   ASNS (ASNS Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. shERK2b-mlpx-puro

   Plasmid

   #65230

   Purpose

   encodes a shRNA against ERK2

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   shRNA against ERK2 (MAPK1 Human)
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAPK1 gRNA (BRDN0001147498)

   Plasmid

   #77789

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAPK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AURKB gRNA (BRDN0001146837)

   Plasmid

   #77619

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human AURKB

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   AURKB (AURKB Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. shERK2b-mlpx-neo

   Plasmid

   #65231

   Purpose

   encodes a shRNA against ERK2

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   shRNA against ERK2 (MAPK1 Human)
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXS-IRES-BLAST flag-CAD 3R-3E

   Plasmid

   #188148

   Purpose

   Expresses C-terminal flag-tagged human CAD 3R-3E in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

   Plasmid

   #195502

   Purpose

   Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA (SOX17 Human)
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(BCL11B-E4(44))-PGKpuro2ABFP-W

    Plasmid

    #208560

    Purpose

    Lentiviral vector expressing gRNA targeting human BCL11B-E4

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    BCL11B-E4(44) (BCL11B Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(BCL11B-E2(24))-PGKpuro2ABFP-W

    Plasmid

    #208555

    Purpose

    Lentiviral vector expressing gRNA targeting human BCL11B-E2

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    BCL11B-E2(24) (BCL11B Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXS-IRES-BLAST flag-CAD 3E@3R

    Plasmid

    #188149

    Purpose

    Expresses C-terminal flag-tagged human CAD 3E@3R in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDH-V2-rCD20-BSD

    Plasmid

    #209753

    Purpose

    To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Mutation

    The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. eScaf

    Bacterial Strain

    #220921

    Purpose

    cssDNA production strain

    Depositor

    Shawn Douglas

    Article

    Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

15. TB205 △trpC

    Bacterial Strain

    #230038

    Purpose

    Fluorescently labelled (mCherry) E. coli, auxotrophic for tryptophan

    Depositor

    Olga Schubert, Martin Ackermann

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

16. TB204 △trpC

    Bacterial Strain

    #230037

    Purpose

    Fluorescently labelled (GFP) E. coli, auxotrophic for tryptophan

    Depositor

    Olga Schubert, Martin Ackermann

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

17. E. coli BW25113 ΔtnaA ΔtrpR

    Bacterial Strain

    #205015

    Purpose

    Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

    Depositor

    Yajun Yan

    Article

    Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available Since

    Aug. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits