We narrowed to 860 results for: golden gate assembly

1. pD(Ct)_CPD-His10

   Plasmid

   #223890

   Purpose

   4G-cloning donor for C-terminal CPD-His10 tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   CPD-His10
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pD(Ct)_3C-TS

   Plasmid

   #223892

   Purpose

   4G-cloning donor for C-terminal 3C-TwinStrep tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3C-TwinStrep
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pD(Gib)_5'alpha

   Plasmid

   #223863

   Purpose

   4G-cloning donor for 5'-Gibson overhang, alpha-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   5'alpha sequence and spacer
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pD(Gib)_5'gamma

   Plasmid

   #223865

   Purpose

   4G-cloning donor for 5'-Gibson overhang, gamma-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   5'gamma sequence and spacer
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pD(Gib)_5'beta

   Plasmid

   #223864

   Purpose

   4G-cloning donor for 5'-Gibson overhang, beta-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   5'beta sequence and spacer
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pD(Gib)_5'delta

   Plasmid

   #223866

   Purpose

   4G-cloning donor for 5'-Gibson overhang, delta-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   5'delta sequence and spacer
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pD(Gib)_3'beta

   Plasmid

   #223867

   Purpose

   4G-cloning donor for 3'-Gibson overhang, beta-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3'beta sequence and spacer
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pD(Gib)_3'gamma

   Plasmid

   #223868

   Purpose

   4G-cloning donor for 3'-Gibson overhang, gamma-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3'gamma sequence and spacer
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pD(Gib)_3'delta

   Plasmid

   #223869

   Purpose

   4G-cloning donor for 3'-Gibson overhang, delta-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3'delta sequence and spacer
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Rich-pT7GG

    Plasmid

    #108443

    Purpose

    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assembly

    Depositor

    Richard Bélanger

    Type

    Empty backbone

    Use

    Xenopus oocyte expression system

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. C39m

    Plasmid

    #120972

    Purpose

    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ccdB toxin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site removed

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. C64m

    Plasmid

    #120980

    Purpose

    MoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).

    Depositor

    Richard Murray

    Insert

    temperature sensitive lambda repressor (cI857)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C35m

    Plasmid

    #120969

    Purpose

    MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).

    Depositor

    Richard Murray

    Insert

    RhlR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BbsI sites removed; amino acid sequence …

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. UC20m

    Plasmid

    #121040

    Purpose

    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).

    Depositor

    Richard Murray

    Insert

    gN2 Cas9 gRNA UC part
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C71m

    Plasmid

    #120983

    Purpose

    MoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam Meyer's improved CinR activator from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).

    Depositor

    Richard Murray

    Insert

    CinR Adam Meyer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. T2m

    Plasmid

    #121029

    Purpose

    MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank file

    Depositor

    Richard Murray

    Insert

    ECK120033736 (164.6x Voigt terminator), short attachment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. T7m

    Plasmid

    #121030

    Purpose

    MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    S. pyogenes tracrRNA terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. T13m

    Plasmid

    #121032

    Purpose

    MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ECK120033737 (second strongest natural Voigt terminator)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C105m

    Plasmid

    #121008

    Purpose

    MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    sfYFP-AAV ssrA (has bbsI)
    

    Use

    Synthetic Biology

    Tags

    AAV degradation tag for ssrA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C106m

    Plasmid

    #121009

    Purpose

    MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    sfYFP-DAS ssrA (has bbsI)
    

    Use

    Synthetic Biology

    Tags

    DAS degradation tag for ssrA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge