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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,461 results for: golden gate assembly

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Showing: 521 - 540 of 1461 results

  1. U7m

    Plasmid
    #121020
    Purpose
    MoClo golden gate assembly BC part for BCD16 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD16
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121020-plasmid-map-sequence-id-236180

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  2. U8m

    Plasmid
    #121021
    Purpose
    MoClo golden gate assembly BC part for BCD24 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD24
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121021-plasmid-map-sequence-id-236184

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  3. C84m

    Plasmid
    #120995
    Purpose
    MoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 28
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120995-plasmid-map-sequence-id-236105

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  4. C95m

    Plasmid
    #121001
    Purpose
    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7 RNA polymerase (Andy please check!)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121001-plasmid-map-sequence-id-236130

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  5. C63m

    Plasmid
    #120979
    Purpose
    MoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lacI
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120979-plasmid-map-sequence-id-236033

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  6. C3m

    Plasmid
    #120956
    Purpose
    MoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)
    Depositor
    Richard Murray
    Insert
    sfGFP
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120956-plasmid-map-sequence-id-235970

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  7. P30m

    Plasmid
    #120942
    Purpose
    MoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pL-lacO1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120942-plasmid-map-sequence-id-238038

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  8. (P2)RBSS7

    Plasmid
    #251372
    Purpose
    RBS originally used in pDF-lac [74]
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S7
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251372-plasmid-map-sequence-id-503741

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  9. (P6)RBSS6

    Plasmid
    #251391
    Purpose
    Synthetic RBS used in expression in Synechocystis
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S6
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251391-plasmid-map-sequence-id-503746

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  10. (P6)RBSS5

    Plasmid
    #251390
    Purpose
    RBS from rbcL gene; slr0090 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S5
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251390-plasmid-map-sequence-id-503745

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  11. (P6)RBSS7

    Plasmid
    #251392
    Purpose
    RBS originally used in pDF-lac [74]
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S7
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251392-plasmid-map-sequence-id-503744

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  12. (P2)RBSS6

    Plasmid
    #251371
    Purpose
    Synthetic RBS used in expression in Synechocystis
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S6
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251371-plasmid-map-sequence-id-503743

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  13. (P2)RBSS5

    Plasmid
    #251370
    Purpose
    RBS from rbcL gene; slr0090 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S5
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251370-plasmid-map-sequence-id-503727

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  14. (P4)RBSS6

    Plasmid
    #251381
    Purpose
    Synthetic RBS used in expression in Synechocystis
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S6
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251381-plasmid-map-sequence-id-503715

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  15. (P4)RBSS7

    Plasmid
    #251382
    Purpose
    RBS originally used in pDF-lac [74]
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S7
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251382-plasmid-map-sequence-id-503712

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  16. (P4)RBSS5

    Plasmid
    #251380
    Purpose
    RBS from rbcL gene; slr0090 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S5
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251380-plasmid-map-sequence-id-503680

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Showing: 521 - 540 of 1461 results