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pBK1297-AAV-EFSNC-dCjCas9-HP1b(79-173)

Plasmid

#223142

Purpose

Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 79-173

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

Plasmid

#223143

Purpose

Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 111-173

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1308-AAV-EFSNC-dSaCas9-HP1bNH(111-173)

Plasmid

#223153

Purpose

Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 111-173

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)

Plasmid

#223152

Purpose

Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 79-173

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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mAzGreen-Cyclin D3-NeoR

Plasmid

#215061

Purpose

Expresses mAzGreen-Cyclin D3 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND3 (CCND3 Human)

Tags

mAzGreen

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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mAzGreen-Cyclin D2-NeoR

Plasmid

#215062

Purpose

Expresses mAzGreen-Cyclin D2 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND2 (CCND2 Human)

Tags

mAzGreen

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SS-Cyclin D2

Plasmid

#215115

Purpose

Expresses SS-Cyclin D2 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND2 (CCND2 Human)

Tags

SS (STREP-STREP)

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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mVenus-Cyclin D1-NeoR

Plasmid

#215068

Purpose

Expresses mVenus-Cyclin D1 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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mAzGreen-Cyclin D1-NeoR

Plasmid

#215060

Purpose

Expresses mAzGreen-Cyclin D1 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Tags

mAzGreen

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mAzGreen-Cyclin D1-PuroR

Plasmid

#215056

Purpose

Expresses mAzGreen-Cyclin D1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

mAzGreen

Expression

Mammalian

Promoter

LTR

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mKO2-CDK6

Plasmid

#215066

Purpose

Expresses mKO2-CDK6 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDK6 (CDK6 Human)

Use

Retroviral

Tags

mKO2

Expression

Mammalian

Promoter

LTR

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mAzGreen-Cyclin D2-PuroR

Plasmid

#215058

Purpose

Expresses mAzGreen-Cyclin D2 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

mAzGreen

Expression

Mammalian

Promoter

LTR

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mAzGreen-Cyclin D3-PuroR

Plasmid

#215059

Purpose

Expresses mAzGreen-Cyclin D3 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

mAzGreen

Expression

Mammalian

Promoter

LTR

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mPlum-PCNA-PuroR

Plasmid

#215046

Purpose

Expresses mPlum-PCNA in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Retroviral

Tags

mPlum

Expression

Mammalian

Promoter

LTR

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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mPlum-PCNA-HygroR

Plasmid

#215047

Purpose

Expresses mPlum-PCNA in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Retroviral

Tags

mPlum

Expression

Mammalian

Promoter

LTR

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-POLB

Plasmid

#215138

Purpose

Expresses mCherry-POLB in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

POLB (POLB Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NEMF_CDKL1

Plasmid

#205867

Purpose

Express mEGFP-tagged fusion protein, NEMF_CDKL1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NEMF_CDKL1 (NEMF, CDKL1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUb FLAG-Pp2A-29B

Plasmid

#195066

Purpose

Expresses FLAG-tagged Drosophila Pp2A-29B protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Protein phosphatase 2A at 29B (Pp2A-29B Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ABR_DUXA

Plasmid

#205767

Purpose

Express mEGFP-tagged fusion protein, ABR_DUXA from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ABR_DUXA (ABR, DUXA Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF3279

Plasmid

#144755

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

LMO1 (LMO1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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