We narrowed to 7,669 results for: Ski

1. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

   Plasmid

   #101060

   Purpose

   AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-PEmax

   Plasmid

   #187893

   Purpose

   All-in-one prime editor piggyBac transposon with PEmax

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   BPSV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pgRNA-CKB

   Plasmid

   #73501

   Purpose

   gRNA expression vector (with mKate2)

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   mKate2-T2A-Bsd
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR v2-sgSLC7A11/xCT-1

   Plasmid

   #161818

   Purpose

   knock out SLC7A11/xCT in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

   Insert

   SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-HyPer7-MEM

   Plasmid

   #136465

   Purpose

   Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   Farnselation tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHTN_HaloTag_KIF5B

   Plasmid

   #213404

   Purpose

   Full-length human kinesin-1 with HaloTag at the N-terminus

   Depositor

   Jonas Ries

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   HaloTag-KIF5B (KIF5B Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUAST-pHuji-GINKO2

   Plasmid

   #177117

   Purpose

   Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

   Depositor

   Robert Campbell

   Article

   Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

   Insert

   pHuji-GINKO2
   

   Expression

   Insect

   Promoter

   hsp70 promoter

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCX-DI-2A

   Plasmid

   #74671

   Purpose

   Mammalian expression of DI-2A (hCD73/F2A/hCD39)

   Depositor

   Roberto Giovannoni

   Article

   De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

   Inserts

   CD73 (NT5E Human)
   CD39 (ENTPD1 Human)
   

   Tags

   F2A

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-mTurquoise2-Caspase-1-mNeonGreen

   Plasmid

   #166784

   Purpose

   FRET analysis (pro caspase-1 cleavage) in living cells

   Depositor

   Hao Wu

   Article

   Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

   Insert

   mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pESF140_HisSpy_DDB1∆B

    Plasmid

    #124213

    Purpose

    Insect cell expression vector for His6, Spy tagged hsDDB1∆B

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    DNA damage-binding protein 1 (DDB1 Human)
    

    Tags

    His6-Spy

    Expression

    Insect

    Mutation

    internal deletion of BPB domain to aid crystalliz…

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-mTurquoise2-IL-1beta-mNeonGreen

    Plasmid

    #166783

    Purpose

    FRET analysis (pro-IL-1beta cleavage) in living cells

    Depositor

    Hao Wu

    Article

    Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

    Insert

    mTurquoise2-IL-1b-mNeongreen (IL1B Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6_HEK3+1T>A_(PP7-C4-Q1)

    Plasmid

    #232437

    Purpose

    pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.hSynap.iAChSnFR

    Plasmid

    #137950

    Purpose

    Expresses iAChSnFR under hSynapsin promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Has Service

    AAV1

    Insert

    iAChSnFR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynanpsin

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDY1623 NOLC1 sgRNA 2

    Plasmid

    #234834

    Purpose

    sgRNA 2 for NOLC1 STITCHR insertion

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUW-TetO-Klf5

    Plasmid

    #128830

    Purpose

    dox-dependent expression of mouse Klf5

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Klf5 (Klf5 Mouse)
    

    Use

    Lentiviral

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6_HEK3_nsgRNA(PP7)

    Plasmid

    #232444

    Purpose

    nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPR v2-sgSLC7A11/xCT-2

    Plasmid

    #161819

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid

    #73499

    Purpose

    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDY1622 NOLC1 sgRNA 1

    Plasmid

    #234833

    Purpose

    sgRNA 1 for NOLC1 STITCHR insertion

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC

    Plasmid

    #229545

    Purpose

    Lentiviral construct to express rtTA and SKM proteins

    Depositor

    Domitilla Del Vecchio

    Article

    Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

    Insert

    rTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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