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pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

Plasmid

#182352

Purpose

Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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GAPDH-CPG-TV

Plasmid

#70148

Purpose

a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh

Use

Mouse Targeting and Synthetic Biology

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSRE SSG

Plasmid

#127692

Purpose

Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Structural Genome

Expression

Mammalian

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CAG-RiboL1-jGCaMP8s

Plasmid

#192611

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RiboL1-jGCaMP8f

Plasmid

#192612

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

Plasmid

#170712

Purpose

dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Inserts

mNeonGreen
mScarlet

Use

Lentiviral

Expression

Mammalian

Promoter

TNNT2 and aMHC

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RiboL1-jGCaMP8m

Plasmid

#192613

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IRES-Hygro

Plasmid

#102423

Purpose

Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

Depositor

Kian Peng Koh

Article

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Insert

tetracyclin-transactivator (rtTA)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Red Glifon Nega

Plasmid

#163117

Purpose

Red fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cells

Depositor

Takashi Tsuboi

Article

Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

Insert

Red Glifon Nega

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hSYN-eGFP

Plasmid

#162512

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

hSYN

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG::Cytbow

Plasmid

#158995

Purpose

Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Cytbow

Tags

The default expressed EBFP2 is fused to H2B

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD2

Plasmid

#20184

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 2 intracellular domain (Notch2 Mouse)

Tags

3X FLAG

Expression

Mammalian

Mutation

Contains murine Notch 2 Intracellular Domain (Met…

Promoter

CMV

Available Since

Jan. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pSH424-ssr

Plasmid

#198026

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Sm resistance cassette

Expression

Bacterial

Promoter

SmR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Cdc42-2G

Plasmid

#68814

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Tags

His and Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-HiBiT-CD81

Plasmid

#162593

Purpose

N-terminally HiBiT-tagged human CD81 under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

human CD81 (CD81 human, Human, Synthetic)

Tags

HiBiT

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Brachyury-eGFP

Plasmid

#21063

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Brachyury promoter

Use

Lentiviral

Tags

eGFP

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-PASS

Plasmid

#193970

Purpose

Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Article

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-ASC

Plasmid

#73952

Purpose

Express c-Myc-tagged human ASC in mammalian cells

Depositor

Christian Stehlik

Article

Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFPN3-5ht6

Plasmid

#35624

Depositor

Kirk Mykytyn

Article

Berbari et al Mol Biol Cell. 2008 Apr;19(4):1540-7. Ep

Insert

5ht6 (Htr6 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pDestTol2CG2--3.5ubb:plin2-tdtomato

Plasmid

#172534

Purpose

Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

-3.5ubb:plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F and R415H mutations obse…

Promoter

zebrafish ubb

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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