We narrowed to 11,563 results for: Vars;

1. pEMS2115

   Plasmid

   #49140

   Purpose

   The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   ssAAV-Ple155-emGFP-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MiniPromoter Ple155 from the human PCP2 gene

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGEX-4T-1_RAF1 (51-131)

   Plasmid

   #119218

   Purpose

   purification of GST-RAF1 RBD from E. coli

   Depositor

   Helen Morrison

   Article

   Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

   Insert

   RAF1 aa 51-131 (RAF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-eNme2-T.1-ABE8e

   Plasmid

   #185668

   Purpose

   Expresses eNme2-T.1-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-T.2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

   Promoter

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. GLuc-PM

   Plasmid

   #164783

   Purpose

   Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
   

   Use

   Tags

   GLuc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CoV2-Spike-EF1a-D614G-N501Y

   Plasmid

   #177939

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (D614G, N501Y) (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G, N501Y

   Promoter

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194381

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB1-A11(2-196, D40G)-Strep

   Plasmid

   #196210

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   D40G

   Promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. GB1-A11(2-196, G38R)-Strep

   Plasmid

   #196209

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   G38R

   Promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETcon_SARS-CoV-2_RBD

   Plasmid

   #166782

   Purpose

   Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

   Depositor

   Jesse Bloom

   Article

   Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

   Insert

   Spike receptor binding domain (RBD) from SARS-CoV-2 (S )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. NanoLuc-PM-Venus

    Plasmid

    #164786

    Purpose

    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
    

    Use

    Tags

    NanoLuc and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57.1 DOX off blast NFS1

    Plasmid

    #160801

    Purpose

    Express Dox repressible NFS1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    NFS1 (NFS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. METTL7A pEGFP-N2 (890)

    Plasmid

    #62017

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    METTL7A (METTL7A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

    Plasmid

    #216477

    Purpose

    lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    dsRed
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXS-IRES-Blast POLA1

    Plasmid

    #160807

    Purpose

    Express POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLA1 (POLA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Codon Optimized

    Promoter

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GB1-A11(2-196, G175R)-Strep

    Plasmid

    #196211

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G175R

    Promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GB1-A11(2-196, G189E)-Strep

    Plasmid

    #196212

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G189E

    Promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro

    Plasmid

    #192684

    Purpose

    Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human IL1RN activating gRNAs #1,2,3 (IL1RN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry N1 MFN2

    Plasmid

    #157759

    Purpose

    Expression of MFN2:mcherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    MFN2 (MFN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmCherry C1 Omp25

    Plasmid

    #157758

    Purpose

    Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    SYNJ2bp (SYNJ2BP Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HDM_Spikedelta21_sinobiological_B.1.617.2

    Plasmid

    #204149

    Purpose

    Expression plasmid for SARS-CoV-2 Delta variant spike

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    SARS-CoV-2 Delta variant spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    21 amino acid cytoplasmic tail deletion

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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