We narrowed to 82,966 results for: ELL

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pcDNA3.1(+)_Pmel-1_TCRA

Plasmid

#175287

Purpose

Expresses Pmel-1 TCRA alpha chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor alpha chain (Tcra Mouse)

Use

Suitable for ivt

Expression

Mammalian

Promoter

CMV and T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-pur-CAG-mCherry

Plasmid

#159458

Purpose

AAVS1 targeting donor plasmid with mCherry expression cassette and puromycin selection gene

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23-Fosprom

Plasmid

#188113

Purpose

Expresses luciferase under control of Fos promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

Insert

Fos promoter (Fos Mouse)

Use

Luciferase

Promoter

Fos endogenous promoter

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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CRISPseq-BFP-backbone

Plasmid

#85707

Purpose

Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

Depositor

Ido Amit

Article

Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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BECLIN-HA WT

Plasmid

#46993

Purpose

Expresses Beclin1 in mammalian cells

Depositor

Kunliang Guan

Article

Russell et al Nat Cell Biol. 2013 Jul;15(7):741-50. do

Insert

becn1 (Becn1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

Plasmid

#86843

Purpose

HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

Depositor

Thomas Cech

Article

Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

Insert

FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)

Tags

FLAG-HaloTag

Expression

Mammalian

Promoter

Endogenous TERT promoter

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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CABE-T3.1

Plasmid

#193267

Purpose

Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T3.1

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLacZ-1735

Plasmid

#115454

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shLacZ-1735

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-HOXA9

Plasmid

#51302

Purpose

Expression of HOXA9 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

homeobox A9 (HOXA9 Human)

Use

Lentiviral

Tags

n/a

Mutation

n/a

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pM-TagRFP657

Plasmid

#176563

Purpose

Expression of TagRFP657 for auxotrophic selection in the absence of methionine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

TagRFP657

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-Eevee-ROCK-NES

Plasmid

#209918

Purpose

A FRET biosensor for ROCK activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

Eevee-ROCK-NES

Use

Lentiviral

Tags

ECFP and YPet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-mSOG-FRB-IRES-mCherry

Plasmid

#136602

Purpose

Encodes FRB tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

N-mSOG-FRB

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28:LanII

Plasmid

#225066

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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NAE1 G8.3 gRNA

Plasmid

#90777

Purpose

3rd generation lentiviral gRNA plasmid targeting human NAE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NAE1 (Guide Designation G8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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BRCA2 A12.2 gRNA

Plasmid

#90551

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A12.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 merlin 1-595

Plasmid

#11623

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
EZH2 E9.3 gRNA

Plasmid

#90684

Purpose

3rd generation lentiviral gRNA plasmid targeting human EZH2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

EZH2 (Guide Designation E9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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RRM1 E1.3 gRNA

Plasmid

#90880

Purpose

3rd generation lentiviral gRNA plasmid targeting human RRM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RRM1 (Guide Designation E1.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FIH1

Plasmid

#21403

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

HIF1AN (HIF1AN Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
EZH2 E10.3 gRNA

Plasmid

#90685

Purpose

3rd generation lentiviral gRNA plasmid targeting human EZH2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

EZH2 (Guide Designation E10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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