We narrowed to 23,456 results for: SHE

1. pENTR221 UBR5 ∆UBR

   Plasmid

   #81063

   Purpose

   Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   W1235L (bp g3704t)

   Promoter

   N/A

   Available since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-Tet-On-Arl13b-RhoA sensor

   Plasmid

   #189613

   Purpose

   Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   Arl13b
   

   Use

   Tags

   GFP and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Tet3G

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR221 UBR5 ∆HECT

   Plasmid

   #81065

   Purpose

   Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   C2768A (bp t8302g and g8303c)

   Promoter

   N/A

   Available since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MmEos3.2

   Plasmid

   #203754

   Purpose

   Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   Src15 n-term (SRC Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SHC003BSD-EXOC7

   Plasmid

   #133298

   Purpose

   Expresses EXOC7 in mammalian cells

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Insert

   Exocyst complex component 7 (EXOC7 Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Syn1-GRAB-HTR6-cilia

   Plasmid

   #189615

   Purpose

   Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   HTR6_GRAB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Syn1

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. plv-AcGFP-SOD1 (G93A)

   Plasmid

   #27142

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Lentiviral

   Tags

   AcGFP

   Expression

   Mutation

   G93A

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

8. Chr1q_Cassette-Integration_gRNA

   Plasmid

   #195126

   Purpose

   gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Chr1q gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet

   Plasmid

   #216386

   Purpose

   Bacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)

   Depositor

   William Weis

   Article

   Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

   Insert

   Dishevelled-2 (Dvl2 Mouse)
   

   Use

   Tags

   6xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet

    Plasmid

    #216387

    Purpose

    Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Dishevelled-2 (Dvl2 Mouse)
    

    Use

    Tags

    6xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-TetOne-Puro-PFDN2

    Plasmid

    #202664

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    prefoldin subunit 2 (PFDN2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXB7 (human) FLAG pSP65

    Plasmid

    #8537

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pF144 pAcGFP1 SOD1G85R

    Plasmid

    #26405

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G85R

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

14. mEos3.2trCD45

    Plasmid

    #203753

    Purpose

    Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD45 (PTPRC Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-NSD3-short

    Plasmid

    #72552

    Purpose

    expresses 3*FLAG tagged human NSD3-short

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEos3.2FP

    Plasmid

    #203757

    Purpose

    Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    NRas c-term (NRAS Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGV3319

    Plasmid

    #170277

    Purpose

    Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    Bst-LF D720A
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    D720A

    Promoter

    T7-lac

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-CoV2-Omicron BA.1 spike

    Plasmid

    #194602

    Purpose

    Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

    Depositor

    Ben Murrell

    Article

    Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

    Insert

    CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FusionRed-Fibrillarin-7

    Plasmid

    #56118

    Purpose

    Localization: Nucleoli, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. OR2M4_Deletion_gRNA

    Plasmid

    #195194

    Purpose

    dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    OR2M4 dual gRNA (OR2M4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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