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pJH4687

Plasmid

#200783

Purpose

Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ins-22 (ins-22 Nematode)

Tags

pHiuorin

Expression

Worm

Promoter

Pnpr-4

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

Plasmid

#119074

Purpose

Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:MCP-p65-HSF1-2a-Cerulean

Expression

Bacterial

Promoter

Zebrafish ubiquitin promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

Plasmid

#119075

Purpose

Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:Com-KRAB-2a-Cerulean

Expression

Bacterial

Promoter

Zebrafish ubiquitin promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-com-Ds

Plasmid

#119077

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xCom-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-MS2-Ds

Plasmid

#119076

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xMS2-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Sec61B

Plasmid

#120244

Purpose

Expresses V5-tagged Sec61B in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Sec61B (Sec61b Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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tdEos-VSVG-16

Plasmid

#57693

Purpose

Localization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

VSVG

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-3xHAtag-SP-PTPRO

Plasmid

#191696

Purpose

For expression coding sequencing of PTPRO

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

signal peptide, 3xHAtag and full length coding sequence of PTPRO

Expression

Mammalian

Mutation

WT

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pPMVAugw

Plasmid

#98300

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

TEFM1EfmvaE>TEBS1

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFP97

Plasmid

#83551

Purpose

Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

CYC1 promoter-yEGFP

Expression

Yeast

Promoter

CYC1

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Nxph4-HA

Plasmid

#128102

Purpose

Express HA tagged Nxph4

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nxph4 (Nxph4 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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p3×FLAG-CMV-14-LanCL2

Plasmid

#73391

Purpose

Expresses FLAG tagged LanCL2

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

LanCL2 (LANCL2 Human)

Tags

3x FLAG tagged

Expression

Mammalian

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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MCV-Rep-

Plasmid

#32058

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-Rep- genome

Mutation

Single cytosine to adenine (C/A) mutation at 2,07…

Promoter

none from vector. Insert contains native promoter…

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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2-Luc-Del-50

Plasmid

#21730

Depositor

Wafik El-Deiry

Article

Zeng et al Nat Genet. 1997 Jan . 15(1):78-82.

Insert

p21 promoter deletion mutant (CDKN1A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Identical to 2-Luc except that the 50-bp region b…

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-SP-CLSTN2-3xHAtag

Plasmid

#191693

Purpose

For expression coding sequencing of CLSTN2

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

signal peptide, full length coding sequence of CLSTN2 and 3xHAtag

Expression

Mammalian

Mutation

WT

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pPIGA-PHD

Plasmid

#26778

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PGK-Hyg-p(A) (PGK1 )

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-Syk-tSH2

Plasmid

#111269

Purpose

express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJKW1813

Plasmid

#174003

Purpose

Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

Depositor

Jing-Ke Weng

Article

Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

Inserts

PpCYP90G4
ATR1 (ATR1 Mustard Weed)

Expression

Yeast

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Hiv7CMV-Cremyc-2A-GFP

Plasmid

#117148

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Article

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Use

Lentiviral

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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FUP95PDZGW (C5)

Plasmid

#74021

Purpose

lentiviral expression of Psd95-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

PDZ

Promoter

hUbC

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only