We narrowed to 25,510 results for: CHI

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LentiU6-hIL1RN gRNA_1-MS2-Puro

Plasmid

#192677

Purpose

Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNA #1 (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p8322 pDONR ATG-YAP1 S94A-Stop

Plasmid

#184526

Purpose

Subcloning YAP1 S94A

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S94A (YAP1 Human)

Use

Unspecified

Mutation

S94A

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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p8321 pDONR ATG-YAP1 S127A-Stop

Plasmid

#184525

Purpose

Subcloning YAP1 S127A

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S127A (YAP1 Human)

Use

Unspecified

Mutation

S127A

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Topo-Tbxas1_ISH_S

Plasmid

#170307

Purpose

Encoding partial sense strand of the mouse Tbxas1 gene for in situ hybridization.

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

Partial sense strand of the mouse Tbxas1 gene for in situ hybridization

Use

For ivf

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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Topo-Tbxas1_ISH_AS

Plasmid

#170308

Purpose

Encoding partial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

Partial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization

Use

For ivf

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM L368G

Plasmid

#169985

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, L368G

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM R394E

Plasmid

#169987

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, R394E

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM F405G

Plasmid

#169988

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, F405G

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM F370G

Plasmid

#169986

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, F370G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND F405G + S297A

Plasmid

#169974

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

S297A, F405G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND E274R + S297A

Plasmid

#169968

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

S297A, E274R

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND R394E + S297A

Plasmid

#169972

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

S297A, R394E

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM E274R

Plasmid

#169977

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM, E274R

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-optimized E6AP F727D

Plasmid

#165101

Purpose

ecoli optimized expression of a GST fusion of Human E6AP F727D

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2014 Jan 10;289(2):1033-48.

Insert

E6AP F727D (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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Oct4deltaPE-53BP1mCherry

Plasmid

#141122

Purpose

Construct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells

Depositor

John Schimenti

Article

Bloom et al Genesis. 2020 Aug;58(8):e23368. doi: 10.

Insert

Fragment of human 53BP1 (TP53BP1 Human)

Use

Mouse Targeting

Tags

mCherry

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mutNSDHL

Plasmid

#136428

Purpose

Expresses NSDHL harboring all currently known mutations within exons 4 and 6 associated with CHILD syndrome.

Depositor

Antonio Amelio

Article

Getz et al Oral Surg Oral Med Oral Pathol Oral Radi

Insert

human NSDHL with mutations in exon 4 and exon 6 (NSDHL Human, Synthetic)

Tags

V5-His tag

Expression

Mammalian

Mutation

C>T = A105V; G>C = A182P; G>A = R199H; G…

Promoter

CMV

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCHKU17.1-2.2

Plasmid

#115530

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pZS1[TreR-L,LacI-L]

Plasmid

#60760

Purpose

Contains PI driving expression TreR-L, and PI driving expression of LacI-L

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

TreR-L
Dimeric LacI

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with TreR L…

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-L,LacI-L]

Plasmid

#60757

Purpose

Contains PIq driving expression RbsR-L, and PI driving expression of LacI-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

RbsR-L
Dimeric LacI

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with RbsR L…

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-NFIA

Plasmid

#141403

Purpose

Inducible Expression of NFIA

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

NFIA (NFIA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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