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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,556 results for: des.1

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Showing: 5521 - 5540 of 66556 results

  7. pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

    Plasmid
    #197454
    Purpose
    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Article
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 Delta-WD40 propeller (FBXW7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW& dimerization domain deleted.
    Promoter
    CMV
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197454-plasmid-map-sequence-id-394916

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  9. pcDNA3.2-V5-DEST-hSFRP1(aa53-166)

    Plasmid
    #189544
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-166.
    Depositor
    Haian Fu
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Expression
    Mammalian
    Mutation
    contains aa53-166
    Available Since
    April 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    189544-plasmid-map-sequence-id-390137

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  10. pDEST27-GST-hSFRP1(aa181-314)

    Plasmid
    #189548
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.
    Depositor
    Haian Fu
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa181-314
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189548-plasmid-map-sequence-id-374132

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  11. pDEST27-GST-hSFRP1(aa53-169)

    Plasmid
    #189545
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.
    Depositor
    Haian Fu
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa53-169
    Available Since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189545-plasmid-map-sequence-id-374145

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  13. pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP

    Plasmid
    #164945
    Purpose
    Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP
    Depositor
    GuangJun Zhang
    Article
    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi
    Insert
    KCNJ13 (KCNJ13 Human)
    Use
    Tol2 transposon destination vector
    Tags
    IRES-EGFP
    Mutation
    Stop codon deleted
    Promoter
    actinb (actb2) promoter
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164945-plasmid-map-sequence-id-325850

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  14. pDestTol2-actinb-kcnj13-Q153H-EGFP

    Plasmid
    #164943
    Purpose
    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154I
    Depositor
    GuangJun Zhang
    Article
    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Tol2 transposon destination vector
    Tags
    EGFP
    Mutation
    Stop codon deleted; Q153H; M154I
    Promoter
    actinb (actb2) promoter
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164943-plasmid-map-sequence-id-325844

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  15. pDestTol2-actinb-kcnj13-T131A-EGFP

    Plasmid
    #164942
    Purpose
    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133R
    Depositor
    GuangJun Zhang
    Article
    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Tol2 transposon destination vector
    Tags
    EGFP
    Mutation
    Stop codon deleted; T131A; G133R
    Promoter
    actinb (actb2) promoter
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164942-plasmid-map-sequence-id-325785

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  19. Desmoplakin I no force control (F40-based)

    Plasmid
    #118726
    Purpose
    The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I (1-1952)-[YPet(short)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1952
    Promoter
    CMV
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118726-plasmid-map-sequence-id-230443

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Showing: 5521 - 5540 of 66556 results