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pLX_TRC208-SYNJ2BP-V5

Plasmid

#92149

Purpose

Expresses V5-tagged human SYNJ2BP in mammalian cells.

Depositor

Alice Ting

Publication

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP-V5 (SYNJ2BP Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK5-FLAG-ITFG2

Plasmid

#87044

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Publication

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ITFG2 (ITFG2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6-DCX-EGFP

Plasmid

#80599

Purpose

pLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152)

Depositor

Torsten Wittmann

Publication

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

Doublecortin (DCX Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PGL4-4X Mut Renilla

Plasmid

#70040

Purpose

Control plasmid for testing 5'UTR folding elements

Depositor

Hans-Guido Wendel

Publication

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Random sequence matched for GC-content

Use

Luciferase

Tags

renilla luciferase

Expression

Mammalian

Promoter

SV40

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-mWnt2b

Plasmid

#42275

Depositor

Chris Garcia, Jeremy Nathans

Publication

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Wnt2b (Wnt2b Mouse)

Expression

Mammalian

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
RSV-betaGal

Plasmid

#24058

Depositor

Herbert Samuels, Frederick Stanley

Publication

Flug et al J Biol Chem. 1987 May 5. 262(13):6373-82

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LacZ (lacZ E. coli)

Tags

RSV promoter

Expression

Mammalian

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
mPERK

Plasmid

#24826

Depositor

Fumihiko Urano

Publication

Ghosh et al PLoS One. 2010 . 5(3):e9575.

Insert

mPERK (Eif2ak3 Mouse)

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pUC57-Rluc-A114-N40

Plasmid

#50561

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Publication

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-A114-N40

Use

Cloning vector

Promoter

lac

Available since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

Plasmid

#84248

Purpose

Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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GCN2-KD1-pGEX4T-1

Plasmid

#21876

Depositor

David Ron

Publication

Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

Insert

GCN2 (Eif2ak4 Mouse)

Tags

GST

Expression

Bacterial

Available since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

Plasmid

#105323

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CAG

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pS148∙CsR

Plasmid

#197858

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Ap/Cb resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMRXIP-3xMyc-STX17FL

Plasmid

#89942

Purpose

Expresses syntaxin17 tagged with Myc in mammalian cells

Depositor

Noboru Mizushima

Publication

Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

Insert

human syntaxin17 (STX17 Human)

Use

Retroviral

Tags

Myc

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLEGFP-K49R-STAT3

Plasmid

#71447

Purpose

Retroviral expression of K49R-STAT3. Please note that this plasmid contains K49R-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Publication

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

K49R

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1

Plasmid

#58432

Purpose

Induces Citrine expression from a promoter containing 1 lexA box in yeast cells

Depositor

Joerg Stelling

Publication

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)1-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-GFPmG1

Plasmid

#50444

Purpose

Expression of a EGFP variant in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFPmG1

Expression

Mammalian

Mutation

E143K, N147Q

Promoter

CAG

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd4-1D4

Plasmid

#42266

Depositor

Chris Garcia, Jeremy Nathans

Publication

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Frizzled 4 (Fzd4 Mouse)

Tags

1D4

Expression

Mammalian

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pRK5-mFzd8-1D4

Plasmid

#42270

Depositor

Chris Garcia, Jeremy Nathans

Publication

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Frizzled 8 (Fzd8 Mouse)

Tags

1D4

Expression

Mammalian

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDuet TDP-43 G294A

Plasmid

#27463

Depositor

Aaron Gitler

Publication

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Glycine 294 mutated to Alanine (G294A)

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
BDS-2 3x MUT (p53 binding site)

Plasmid

#16516

Depositor

Bert Vogelstein

Publication

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

p53 binding site (SFN Human)

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant p53 binding sites

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article