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pLN553 (FuGW-S(MAFK)p-Sponge7)

Plasmid

#105203

Purpose

Module 2 - synthetic promoter MAFK drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(MAFK)p-Sponge7

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRSM15

Plasmid

#101640

Purpose

Encodes the fluorescent protein EG-4 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

EG-4

Tags

6XHis

Expression

Bacterial

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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mKate1.31-CytERM-N-17

Plasmid

#56080

Purpose

Localization: Endoplasmic Reticulum, Excitation: 588, Emission: 635

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

CytERM

Tags

mKate1.31 (aka mKate2)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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CJ40 (let-7 seed mutant perfect site)

Plasmid

#17773

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

let-7 seed mutant perfect site (let-7 Fly)

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

seed mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pAc5.1A-Dm-miR-14

Plasmid

#22763

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 14 (mir-14 Fly, primary transcript)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt8A STOP

Plasmid

#35879

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8A (Wnt8a Human)

Use

Entry vector

Mutation

Silent Mutation A (600) --> G

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-315

Plasmid

#22765

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro-RNA 315 (mir-315 Fly, primary transcript)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pEF-1a_RfxCas13d-HA-IRES-BFP-2A-BSD

Plasmid

#251220

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven RfxCas13d expressing cells

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

RfxCas13d

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-a_LbuCas13a-HA-IRES-BFP-2A-BSD

Plasmid

#251219

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven LbuCas13a expressing cells.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

LbuCas13a

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pEF-1a_dEGFP-Y66S-IRES-PuroR-WPRE

Plasmid

#251210

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven dEGFP-Y66S expressing cells. Puromycin selection marker.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

dEGFP-Y66S

Use

Lentiviral

Expression

Mammalian

Mutation

Y66S

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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Delta6_ftnA-ftnA

Plasmid

#253339

Purpose

Delta6 tagged ferritin

Depositor

Qiangjun Zhou

Article

Luo et al bioRxiv [Preprint]. 2026 Jan 20:2026.01.

Insert

Delta6-ftnA-ftnA (ftnA E. coli)

Expression

Bacterial

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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V12_ftnA-ftnA

Plasmid

#253338

Purpose

V12 tagged ferritin

Depositor

Qiangjun Zhou

Article

Luo et al bioRxiv [Preprint]. 2026 Jan 20:2026.01.

Insert

V12-ftnA-ftnA (ftnA E. coli)

Expression

Bacterial

Promoter

T7

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-GBKT7

Plasmid

#248655

Purpose

Empty vector for generating yeast 2-hybrid GAL4 DNA binding domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 binding domain, Myc

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-GADT7

Plasmid

#248657

Purpose

Empty vector for generating yeast 2-hybrid GAL4 activation domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 activation domain, HA

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pKH032

Plasmid

#224548

Purpose

2A-3xNLS-2xClover mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

2xClover

Use

Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKH040

Plasmid

#224549

Purpose

2A-3xNLS-tdTomato mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

tdTomato

Use

CRISPR and Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1829 OpIE2-Bn

Plasmid

#242438

Purpose

Insect OpIE2 promoter expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Insert

Barnase (J5X95_RS06160 Bacillus amyloliquefaciens)

Expression

Insect

Promoter

OpIE2

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1767 PUb-Bs-TRE-Bn

Plasmid

#242441

Purpose

Ae aegypti PUb promoter expressing barstar, and TRE expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Inserts

Barstar
DsRED
barnase

Expression

Insect

Promoter

AePUb, Hr5/IE1, and TRE

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c26

Plasmid

#242875

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (V122M, A369P, 434G)

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

V122M, A369P, 434G

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c15

Plasmid

#242873

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (V122M, E434G)

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

V122M, E434G

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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